Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas12b is a newly emerged genome engineering system. Here, we compared Cas12b from Alicyclobacillus acidoterrestris (Aac), Alicyclobacillus acidiphilus (Aa), Bacillus thermoamylovorans (Bth) and Bacillus hisashii (Bh) for genome engineering in rice, an important crop. We found AaCas12b was more efficient than AacCas12b and BthCas12b for targeted mutagenesis, which was further demonstrated in multiplexed genome editing. We also engineered the Cas12b systems for targeted transcriptional repression and activation. Our work establishes Cas12b as the third promising CRISPR system, after Cas9 and Cas12a, for plant genome engineering.

簇状规则间隔的短回文重复序列（CRISPR）–Cas12b是一种新兴的基因组工程系统。在这里，我们比较Cas12b从脂环耐热菌（AAC），脂环acidiphilus（AA），芽孢杆菌thermoamylovorans（BTH）诱导和hisashii芽孢杆菌（BH）在水稻基因组工程，一个重要的作物。我们发现AaCas12b在定向诱变方面比AacCas12b和BthCas12b更有效，这在多重基因组编辑中得到了进一步证明。我们还设计了Cas12b系统，用于靶向转录抑制和激活。我们的工作将Cas12b确立为继Cas9和Cas12a之后用于植物基因组工程的第三种有前途的CRISPR系统。