Structural insights into influenza A virus ribonucleoproteins reveal a processive helical track as transcription mechanism.,Nature Microbiology

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Structural insights into influenza A virus ribonucleoproteins reveal a processive helical track as transcription mechanism.
Nature Microbiology ( IF 20.5 ) Pub Date : 2020-03-09 , DOI: 10.1038/s41564-020-0675-3
Rocío Coloma ₁ , Rocío Arranz ₁ , José M de la Rosa-Trevín _{1,

2} , Carlos O S Sorzano ₁ , Sandie Munier _{3,

4,

5} , Diego Carlero ₁ , Nadia Naffakh _{3,

4,

5} , Juan Ortín ₁ , Jaime Martín-Benito ₁

Affiliation

The influenza virus genome consists of eight viral ribonucleoproteins (vRNPs), each consisting of a copy of the polymerase, one of the genomic RNA segments and multiple copies of the nucleoprotein arranged in a double helical conformation. vRNPs are macromolecular machines responsible for messenger RNA synthesis and genome replication, that is, the formation of progeny vRNPs. Here, we describe the structural basis of the transcription process. The mechanism, which we call the 'processive helical track', is based on the extreme flexibility of the helical part of the vRNP that permits a sliding movement between both antiparallel nucleoprotein-RNA strands, thereby allowing the polymerase to move over the genome while bound to both RNA ends. Accordingly, we demonstrate that blocking this movement leads to inhibition of vRNP transcriptional activity. This mechanism also reveals a critical role of the nucleoprotein in maintaining the double helical structure throughout the copying process to make the RNA template accessible to the polymerase.

中文翻译：

对甲型流感病毒核糖核蛋白的结构见解揭示了一种渐进的螺旋轨道作为转录机制。

流感病毒基因组由八个病毒核糖核蛋白（vRNP）组成，每个核糖核酸均由聚合酶的一个拷贝，一个基因组RNA片段和一个双螺旋构象的核蛋白的多个拷贝组成。vRNP是负责信使RNA合成和基因组复制（即子代vRNPs形成）的大分子机器。在这里，我们描述了转录过程的结构基础。我们称其为“过程性螺旋轨道”的机制是基于vRNP螺旋部分的极高灵活性，它允许两条反平行核蛋白-RNA链之间滑动，从而使聚合酶在结合时可在基因组上移动两个RNA末端。因此，我们证明阻止这种运动导致抑制vRNP转录活性。该机制还揭示了核蛋白在整个复制过程中维持双螺旋结构的关键作用，以使RNA模板可用于聚合酶。

更新日期：2020-03-09

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