Bikaverin is a fungal red pigment that presents antimicrobial and antitumor activities. Therefore, this substance could be used as an alternative additive in the food and pharmaceutical industries. The aim of this work was to use response surface methodology to optimize the fermentation conditions and maximize the production of bikaverin in shake flasks. The variables investigated were agitation speed (71–289 rpm), temperature (21–35 °C), and substrate (rice) concentration in the culture medium (16.4–83.6 g/L). The agitation speed had a positive effect on red pigment production, while substrate concentration and temperature had the opposite effect. Maximum bikaverin production was predicted to occur using 289 rpm, 24.3 °C, and 16.4 g/L rice concentration. Experimental validation using 289 rpm, 28 °C, and 20 g/L rice concentration was 6.2% higher than predicted by the model. The present investigation was important for defining the best conditions for the production of bikaverin.

中文翻译：

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模拟尖孢镰刀菌CCT7620在摇瓶培养物中生产比卡维林

碧卡维林是一种真菌红色素，具有抗菌和抗肿瘤活性。因此，该物质可用作食品和制药行业的替代添加剂。这项工作的目的是使用响应表面方法优化发酵条件，并在摇瓶中最大限度地增加比卡韦林的产量。研究的变量是搅拌速度（71–289 rpm），温度（21–35°C）和培养基中底物（大米）的浓度（16.4–83.6 g / L）。搅拌速度对红色颜料的产生有积极影响，而底物浓度和温度则相反。预计在289 rpm，24.3°C和16.4 g / L的大米浓度下将产生最大的双卡维林产量。使用289 rpm，28°C和20 g / L稻米浓度的实验验证为6。比模型预测的结果高2％。目前的研究对于确定生产比卡维林的最佳条件非常重要。