Using a high-throughput sequencing approach, we identified four genomoviruses (family Genomoviridae) associated with a sweet orange (Citrus sinensis) plant collected in Tunisia. The ssDNA genomes of these genomoviruses, which were amplified, cloned and Sanger sequenced, range in size from 2156 to 2191 nt. Three of these viruses share > 99% full-genome pairwise sequence identity and are referred to as citrus Tunisia genomovirus 1 (CTNGmV-1). The CTNGmV-1 isolates share < 62% genome-wide pairwise nucleotide sequence identity with other genomoviruses and belong to the genus Gemykolovirus. The genome of the fourth virus, which was called CTNGmV-2, shares < 68% nucleotide sequence identity with other genomoviruses and belongs to the genus Gemycircularvirus. Based on the species demarcation criteria for members of the family Genomoviridae, CTNGmV-1 and -2 would each represent a new species. Although found associated with Citrus sp. plants, it is likely that these viruses infect fungi or other organisms associated with the plants.

中文翻译：

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代表突尼斯柑橘中鉴定的两种新基因病毒的病毒。

使用高通量测序方法，我们鉴定了四种与突尼斯采集的甜橙（Citrus sinensis）植物相关的基因病毒（Genomoviridae家族）。这些基因病毒的ssDNA基因组经过扩增，克隆和Sanger测序后，大小范围为2156到2191 nt。这些病毒中的三种共享全基因组成对序列同一性> 99％，被称为柑橘突尼斯基因病毒1（CTNGmV-1）。CTNGmV-1分离株与其他基因病毒共享<62％的全基因组成对核苷酸序列同一性，并且属于芽孢病毒属。第四种病毒的基因组称为CTNGmV-2，与其他基因病毒共享<68％的核苷酸序列同一性，属于双螺旋病毒属。根据Genomoviridae家族成员的物种划分标准，CTNGmV-1和-2将各自代表一个新物种。虽然发现与柑橘属。在植物中，这些病毒很可能会感染真菌或与植物相关的其他生物。