Abstract The Innate® potato cultivars, Glaciate, Acclimate, and Hibernate are engineered for bruise and cold sweetening resistance, low acrylamide forming potential and increased Late Blight resistance. Phenotyping trials from 2015 to 2017 characterized the in-field performance, postharvest physiology, and process quality of these cultivars in the Columbia Basin of Washington. Plant growth, tuber yields and specific gravities of Acclimate and Hibernate were comparable to their respective parental cultivars, Ranger Russet and Atlantic. Glaciate exhibited lower yield and specific gravity compared with Russet Burbank. All Innate® cultivars had lower reducing sugars at harvest than their conventional counterparts resulting in superior process fry color. Asparagine (Asn) increased many-fold during development of Russet Burbank and Atlantic tubers but remained relatively low in Innate® tubers due to silenced Asn synthetase. Tuber respiration rates during sequential periods of low temperature sweetening (LTS, 4 °C), reconditioning (16 °C), and subsequent cold storage (4 °C) were largely comparable for Acclimate versus Ranger and for Hibernate versus Atlantic. Glaciate tubers, however, maintained significantly higher respiration rates than Russet Burbank tubers. Silenced invertase conferred resistance to reducing sugar buildup at 4 °C, resulting in superior process quality of the Innate® cultivars regardless of storage temperature. However, cold storage (4 °C) induced substantial increases in Suc concentration of Innate® tubers, which was then lowered 43–73 % by reconditioning at 16 °C. Subsequent storage at 4 °C had no further effect on Suc concentrations, demonstrating the efficacy of reconditioning as a management technique to reduce and minimize Suc levels during prolonged cold storage of Innate® tubers. While heat stress exacerbated the cold-induced buildup of Suc in Innate® tubers and reducing sugars in the parental cultivars, the increases in total sugar (Suc + Glc + Fru) concentrations remained equal, indicating similar elevated levels of starch catabolism during LTS of heat-stressed tubers. However, by virtue of silenced invertase, Innate® tubers were highly tolerant of heat stress for retention of process quality.

中文翻译：

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哥伦比亚盆地种植的工程马铃薯（Solanum tuberosum L.）的发育和收获后生理表型

摘要 Innate® 马铃薯品种 Glaciate、Acclimate 和 Hibernate 具有抗擦伤和抗冷甜味、低丙烯酰胺形成潜力和增强的晚疫病抗性。2015 年至 2017 年的表型试验表征了华盛顿哥伦比亚盆地这些品种的田间表现、收获后生理和加工质量。Acclimate 和 Hibernate 的植物生长、块茎产量和比重与其各自的亲本品种 Ranger Russet 和 Atlantic 相当。与 Russet Burbank 相比，冰川的产量和比重较低。所有 Innate® 品种在收获时的还原糖含量都低于传统品种，从而产生了卓越的加工鱼苗颜色。在 Russet Burbank 和大西洋块茎的发育过程中，天冬酰胺 (Asn) 增加了许多倍，但由于 Asn 合成酶沉默，Innate® 块茎中的天冬酰胺含量仍然相对较低。在低温甜化（LTS，4°C）、修复（16°C）和随后的冷藏（4°C）的连续期间，块茎呼吸速率在很大程度上与适应与游侠以及冬眠与大西洋的相当。然而，冰川块茎的呼吸速率明显高于赤褐色伯班克块茎。沉默的转化酶赋予了在 4 °C 下对还原糖积累的抵抗力，从而使 Innate® 品种无论储存温度如何都具有卓越的加工质量。然而，冷藏 (4 °C) 会导致 Innate® 块茎的 Suc 浓度显着增加，然后通过在 16 °C 下重新调节将其降低 43-73%。随后在 4 °C 下储存对 Suc 浓度没有进一步影响，证明了修复作为一种管理技术的功效，可在 Innate® 块茎的长期冷藏期间降低和最小化 Suc 水平。虽然热应激加剧了冷诱导的 Innate® 块茎中的 Suc 积累和亲本栽培品种中的还原糖，但总糖 (Suc + Glc + Fru) 浓度的增加保持相等，表明在热 LTS 期间淀粉分解代谢水平类似升高- 强调块茎。然而，凭借沉默的转化酶，Innate® 块茎高度耐受热应力以保持加工质量。展示了修复作为一种管理技术的功效，可在 Innate® 块茎的长期冷藏期间降低和最小化 Suc 水平。虽然热应激加剧了冷诱导的 Innate® 块茎中的 Suc 积累和亲本栽培品种中的还原糖，但总糖 (Suc + Glc + Fru) 浓度的增加保持相等，表明在热 LTS 期间淀粉分解代谢水平类似升高- 强调块茎。然而，凭借沉默的转化酶，Innate® 块茎高度耐受热应力以保持加工质量。展示了修复作为一种管理技术的功效，可在 Innate® 块茎的长期冷藏期间降低和最小化 Suc 水平。虽然热应激加剧了冷诱导的 Innate® 块茎中的 Suc 积累和亲本栽培品种中的还原糖，但总糖 (Suc + Glc + Fru) 浓度的增加保持相等，表明在热 LTS 期间淀粉分解代谢水平升高- 强调块茎。然而，凭借沉默的转化酶，Innate® 块茎高度耐受热应力以保持加工质量。表明在热应激块茎的 LTS 期间淀粉分解代谢水平类似升高。然而，凭借沉默的转化酶，Innate® 块茎高度耐受热应力以保持加工质量。表明在热应激块茎的 LTS 期间淀粉分解代谢水平类似升高。然而，凭借沉默的转化酶，Innate® 块茎高度耐受热应力以保持加工质量。