Enzymatic modification is generally applied to enhance desirable attributes of starch. In this study, β-cyclodextrin glycosyltransferase (β-CGTase) together with a specific cyclodextrinase (CDase) was used to modify corn starch. For single β-CGTase treatment, the molecular weight (M_w) was reduced to 36.7 × 10⁵ g/mol and 7.7 × 10⁵ g/mol for 1 h and 6 h reaction, and the debranched samples had a high proportion of chains with degree of polymerization (DP) < 13, indicating an apparent depolymerization effect. CDase could hardly act on starch, while it had a high preference for cyclodextrin hydrolysis and produced mainly maltotetraose with β-CGTase. There was a synergistic effect between β-CGTase and CDase in starch degradation. Interestingly, the utilization of dual-enzyme significantly promoted the retrogradation inhibition property of starch, reducing the retrogradation enthalpy from 5.65 J/g to 1.42 J/g. It was proved that β-CGTase and CDase had a great prospect in the future application.

酶促修饰通常用于增强淀粉的理想特性。在这项研究中，β-环糊精糖基转移酶（β-CGTase）与特定的环糊精酶（CDase）一起用于修饰玉米淀粉。对于单个β-CGTase处理，分子量（M _w）降至36.7×10 ⁵ g / mol和7.7×10 ⁵g / mol反应1 h和6 h，并且脱支的样品具有高比例的链，聚合度（DP）<13，表明有明显的解聚作用。CDase几乎不作用于淀粉，而对环糊精的水解具有很高的偏好，并且主要通过β-CGTase产生麦芽四糖。β-CGTase和CDase在淀粉降解中具有协同作用。有趣的是，利用双重酶显着提高了淀粉的回生抑制性能，使回生焓从5.65 J / g降低到1.42 J / g。证明了β-CGTase和CDase在未来的应用中具有广阔的前景。