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Structural and property characterization of corn starch modified by cyclodextrin glycosyltransferase and specific cyclodextrinase
Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2020-03-09 , DOI: 10.1016/j.carbpol.2020.116137
Hangyan Ji , Yuxiang Bai , Xiaoxiao Li , Danni Zheng , Yu Shen , Zhengyu Jin

Enzymatic modification is generally applied to enhance desirable attributes of starch. In this study, β-cyclodextrin glycosyltransferase (β-CGTase) together with a specific cyclodextrinase (CDase) was used to modify corn starch. For single β-CGTase treatment, the molecular weight (Mw) was reduced to 36.7 × 105 g/mol and 7.7 × 105 g/mol for 1 h and 6 h reaction, and the debranched samples had a high proportion of chains with degree of polymerization (DP) < 13, indicating an apparent depolymerization effect. CDase could hardly act on starch, while it had a high preference for cyclodextrin hydrolysis and produced mainly maltotetraose with β-CGTase. There was a synergistic effect between β-CGTase and CDase in starch degradation. Interestingly, the utilization of dual-enzyme significantly promoted the retrogradation inhibition property of starch, reducing the retrogradation enthalpy from 5.65 J/g to 1.42 J/g. It was proved that β-CGTase and CDase had a great prospect in the future application.



中文翻译:

环糊精糖基转移酶和特异性环糊精酶修饰的玉米淀粉的结构和性质表征

酶促修饰通常用于增强淀粉的理想特性。在这项研究中,β-环糊精糖基转移酶(β-CGTase)与特定的环糊精酶(CDase)一起用于修饰玉米淀粉。对于单个β-CGTase处理,分子量(M w)降至36.7×10 5 g / mol和7.7×10 5g / mol反应1 h和6 h,并且脱支的样品具有高比例的链,聚合度(DP)<13,表明有明显的解聚作用。CDase几乎不作用于淀粉,而对环糊精的水解具有很高的偏好,并且主要通过β-CGTase产生麦芽四糖。β-CGTase和CDase在淀粉降解中具有协同作用。有趣的是,利用双重酶显着提高了淀粉的回生抑制性能,使回生焓从5.65 J / g降低到1.42 J / g。证明了β-CGTase和CDase在未来的应用中具有广阔的前景。

更新日期:2020-03-09
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