Myricetin, a common dietary flavonoid, was reported to possess many different biological activities such as anti-oxidant, anti-inflammatory, and antiviral effects. In this study, we explored the anti-HSV effects and mechanisms of myricetin both in vitro and in vivo. The results showed that myricetin possessed anti-HSV-1 and HSV-2 activities with very low toxicity, superior to the effects of acyclovir. Myricetin may block HSV infection through direct interaction with virus gD protein to interfere with virus adsorption and membrane fusion, which was different from the nucleoside analogues such as acyclovir. Myricetin also down-regulate the cellular EGFR/PI3K/Akt signaling pathway to further inhibit HSV infection and its subsequent replication. Most importantly, intraperitoneal therapy of myricetin markedly improved mice survival and reduced virus titers in both lungs and spinal cord. Therefore, the natural dietary flavonoid myricetin has potential to be developed into a novel anti-HSV agent targeting both virus gD protein and cellular EGFR/PI3K/Akt pathway.

中文翻译：

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杨梅素通过靶向病毒gD蛋白和细胞EGFR / PI3K / Akt途径抑制单纯疱疹病毒。

据报道，杨梅素是一种常见的饮食类黄酮，具有许多不同的生物学活性，例如抗氧化，抗炎和抗病毒作用。在这项研究中，我们探讨了杨梅素在体外和体内的抗HSV效应和机制。结果表明，杨梅素具有抗HSV-1和HSV-2活性，且毒性极低，优于阿昔洛韦。杨桃素可能通过与病毒gD蛋白直接相互作用来阻止HSV感染，从而干扰病毒吸附和膜融合，这与核苷类似物（例如阿昔洛韦）不同。杨梅素还下调细胞EGFR / PI3K / Akt信号传导途径，以进一步抑制HSV感染及其随后的复制。最重要的是，杨梅素的腹膜内治疗显着提高了小鼠的存活率，并降低了肺和脊髓的病毒滴度。因此，天然饮食类黄酮杨梅素有可能被开发成针对病毒gD蛋白和细胞EGFR / PI3K / Akt途径的新型抗HSV药物。