Tissue engineered vascular grafts (TEVGs) with diameters <6 mm have broad applications. However, maintaining graft post-implantation patency and function is still difficult to achieve and of urgent necessity. In this study, we use an effective strategy to make the large-pore electrospun polycaprolactone (PCL) cotton material (Cotton) and utilized immunoreactive pre-cultivation to prepare a small diameter autologous TEVG. The material assessment via scanning electron microscopy revealed that Cotton has favorable characteristics that make it suitable for immunoreactive pre-cultivation. It also possesses great mechanical properties. Due to strong reshape ability of Cotton, designed vascular patch and vascular scaffold and others could be obtained. For vascular scaffold, Cotton was wrapped around a polytetrafluoroethylene (PTFE) tube and implanted subcutaneously in rats for up to 2 weeks to prepare autologous TEVGs. Autologous TEVGs were then transplanted into the abdominal aorta of the same rat. Cotton TEVG formed functional graft with favorable patency after transplantation for 4 weeks. Histological results showed that the TEVGs had characteristics similar to that of the native aorta. Our results indicate PCL cotton material could be a kind of shapeable material and used to create a promising biocompatible and functional vascular graft over a relatively short period of time.

直径小于6毫米的组织工程化血管移植物（TEVG）具有广泛的应用。但是，保持移植后的通畅性和功能仍然是难以实现的，并且迫切需要。在这项研究中，我们使用一种有效的策略来制造大孔电纺聚己内酯（PCL）棉材料（棉），并利用免疫反应预培养来制备小直径自体TEVG。通过扫描电子显微镜进行的材料评估显示，棉花具有有利的特性，使其适合进行免疫反应性预培养。它还具有出色的机械性能。由于棉花具有很强的重塑能力，因此可以获得设计好的血管斑块和血管支架等。对于血管支架，将棉缠绕在聚四氟乙烯（PTFE）管上，并皮下植入大鼠中长达2周，以制备自体TEVG。然后将自体TEVGs移植到同一只大鼠的腹主动脉中。棉花TEVG移植4周后形成功能良好的移植物，通畅性良好。组织学结果表明，TEVG具有与天然主动脉相似的特征。我们的结果表明，PCL棉材料可以是一种可塑材料，可用于在相对较短的时间内创建有前途的生物相容性和功能性血管移植物。棉花TEVG移植4周后形成功能良好的移植物，通畅性良好。组织学结果表明，TEVG具有与天然主动脉相似的特征。我们的结果表明，PCL棉材料可以是一种可塑材料，可用于在相对较短的时间内创建有前途的生物相容性和功能性血管移植物。棉花TEVG移植4周后形成功能良好的移植物，通畅性良好。组织学结果表明，TEVG具有与天然主动脉相似的特征。我们的结果表明，PCL棉材料可以是一种可塑材料，可用于在相对较短的时间内创建有前途的生物相容性和功能性血管移植物。