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Nanostructured polymer assemblies stabilize photoactivatable anticancer ruthenium complexes under physiological conditions.
Journal of Inorganic Biochemistry ( IF 3.8 ) Pub Date : 2020-03-09 , DOI: 10.1016/j.jinorgbio.2020.111052
Mingjia Chen 1 , Wen Sun 2 , Annika Kretzschmann 1 , Hans-Jürgen Butt 1 , Si Wu 3
Affiliation  

Photoactivatable ruthenium (Ru) complexes are promising compounds for anticancer phototherapy. They must be stable under physiological conditions before they are transported to cancer cells. In this paper, we systematically studied the stabilities of two Ru-containing block copolymers (Ru complexes as side group or in main chain, respectively) and their corresponding Ru complexes in different media, including saline, bovine serum albumin (BSA) solution, Dulbecco's Modified Eagle's Medium (DMEM, pH 6.5) and DMEM (pH 5.5) with 1.0 mM glutathione (GSH). Their stabilities were studied by monitoring the metal-to-ligand charge transfer (MLCT) bands of the Ru moieties via UV-Vis absorption spectroscopy. The MLCT bands of Ru complexes changed to varying degrees within a day in the above-mentioned media, indicating that they were instable. In contrast, the MLCT bands of Ru-containing polymer assemblies did not change significantly under the same conditions. These results showed that the self-assembled nanostructures protected the Ru moieties and improved their stability. In addition, these nanostructured polymer assemblies could be activated by red light. Therefore, the studied Ru-containing polymer assemblies are more suitable for in vivo applications than their low-molecular weight analogues.

中文翻译:

纳米结构的聚合物组件可在生理条件下稳定光活化的抗癌钌络合物。

光活化钌(Ru)复合物是用于抗癌光疗的有前途的化合物。它们必须先在生理条件下稳定,然后才能转移到癌细胞中。在本文中,我们系统地研究了两种含Ru的嵌段共聚物(分别位于侧面或主链上的Ru络合物)及其在不同介质(包括盐水,牛血清白蛋白(BSA)溶液,Dulbecco's介质)中对应的Ru络合物的稳定性。具有1.0 mM谷胱甘肽(GSH)的改良Eagle培养基(DMEM,pH 6.5)和DMEM(pH 5.5)。通过紫外-可见吸收光谱法监测Ru部分的金属-配体电荷转移(MLCT)带,研究了它们的稳定性。在上述介质中,Ru配合物的MLCT带在一天之内变化程度不同,表明它们是不稳定的。相反,在相同条件下,含Ru的聚合物组装体的MLCT带没有明显变化。这些结果表明,自组装的纳米结构保护了Ru部分并提高了其稳定性。另外,这些纳米结构的聚合物组件可以被红光激活。因此,所研究的含Ru的聚合物组件比它们的低分子量类似物更适合于体内应用。
更新日期:2020-03-09
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