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An elegant technology for ultrasensitive impedimetric and voltammetric determination of cholestanol based on a novel molecularly imprinted electrochemical sensor.
Chemistry and Physics of Lipids ( IF 3.4 ) Pub Date : 2020-03-09 , DOI: 10.1016/j.chemphyslip.2020.104895
Ali R Jalalvand 1 , Mohammad Mahdi Zangeneh 2 , Faramarz Jalili 1 , Shokoufeh Soleimani 1 , Jose Manuel Díaz-Cruz 3
Affiliation  

In this work, a novel molecularly imprinted electrochemical sensor (MIES) has been fabricated based on electropolymerization of a molecularly imprinted polymer (MIP) onto a glassy carbon electrode (GCE) modified with gold-palladium alloy nanoparticles (AuPd NPs)/polydopamine film (PDA)/multiwalled carbon nanotubes-chitosan-ionic liquid (MWCNTs-CS-IL) for voltammetric and impedimetric determination of cholestanol (CHO). Modifications applied to the bare GCE formed an excellent biocompatible composite film which was able to selectively detect CHO molecules. Modifications applied to the bare GCE were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (SEM). Under optimal experimental conditions, the sensor was able to detect CHO in the range of 0.1-60 pM and 1-50 pM by EIS and DPV, respectively. Moreover, the sensor showed high sensitivity, selectivity, repeatability, reproducibility, low interference and good stability towards CHO determination. Our records confirmed that the sensor was successfully able to the analysis real samples for determination of CHO.

中文翻译:

基于新型分子印迹电化学传感器的一种用于胆固醇的超灵敏阻抗测定和伏安测定的精巧技术。

在这项工作中,基于分子印迹聚合物(MIP)在金-钯合金纳米粒子(AuPd NPs)/聚多巴胺膜修饰的玻璃碳电极(GCE)上进行电聚合,制得了新型分子印迹电化学传感器(MIES) PDA)/多壁碳纳米管-壳聚糖离子液体(MWCNTs-CS-IL)用于伏安法和阻抗法测定胆固醇(CHO)。对裸露的GCE进行的修饰形成了出色的生物相容性复合膜,该膜能够选择性地检测CHO分子。通过扫描电子显微镜(SEM),循环伏安法(CV)和电化学阻抗谱(SEM)对应用于裸GCE的修饰进行了表征。在最佳实验条件下,该传感器能够通过EIS和DPV检测到0.1-60 pM和1-50 pM的CHO,分别。此外,该传感器显示出高灵敏度,选择性,可重复性,可重复性,低干扰和对CHO测定的良好稳定性。我们的记录证实,该传感器能够成功分析实际样品以确定CHO。
更新日期:2020-03-09
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