The co-presence of more than one mycotoxin in food is being evidenced in last food surveys as reported in the literature. Beauvericin (BEA) is a non-legislated emergent mycotoxin while Ochratoxin A (OTA) has been widely studied and legislated. Concentration range individually studied was from 2.5 to 0.3 μM for BEA and from 25 to 3.1 μM for OTA; binary mixture [BEA + OTA] comprised concentrations of 1:10 ratio from [2.5 + 25] to [3.1 + 0.3] μM. Potential of toxicity of BEA in HepG2 cells was the highest at all times assayed (24, 48 and 72h). LPO was performed through malondyaldehyde (MDA) detection denoting in the binary mixture for [1.25 + 12.5] μM and at 24 and 72h the highest disturbance values. ROS denoted differences respect to the control at different times specially for OTA, while in binary combination only for few point times was denoted. Effects detected for ROS and LPO were connected with alterations detected for glutathione levels of oxidized and reduced form. A real scenario of consumers chronically exposed to different mycotoxins and their mixtures is here presented highlighting the good methodology to assess the risk from exposure to combinations of chemicals in food.

中文翻译：

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白霉素和曲霉毒素A霉菌毒素单独或组合在HepG2细胞中可改变脂质过氧化作用，活性氧水平和谷胱甘肽。

如文献报道，在最近的食物调查中，食物中同时存在一种以上的霉菌毒素。Beauvericin（BEA）是一种非立法的紧急真菌毒素，而O曲毒素A（OTA）已被广泛研究和立法。单独研究的浓度范围对于BEA为2.5至0.3μM，对于OTA为25至3.1μM；二元混合物[BEA + OTA]的浓度为1：2.5，浓度范围为[2.5 + 25]至[3.1 + 0.3]μM。在所有检测的时间（24、48和72h），BEA对HepG2细胞的毒性潜力最高。LPO是通过丙二醛（MDA）检测进行的，表示二元混合物中的浓度为[1.25 + 12.5]μM，并且在24和72h时出现最高干扰值。ROS专门针对OTA表示在不同时间相对于对照的差异，而在二进制组合中仅表示了几个点时间。检测到的ROS和LPO的影响与检测到的氧化型和还原型谷胱甘肽水平的变化有关。本文介绍了消费者长期暴露于不同霉菌毒素及其混合物的真实情况，强调了评估暴露于食物中化学物质组合的风险的良好方法。