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Bioaugmented constructed wetlands for denitrification of saline wastewater: A boost for both microorganisms and plants
Environment International ( IF 10.3 ) Pub Date : 2020-03-08 , DOI: 10.1016/j.envint.2020.105628
Xinyi Wang , Hui Zhu , Baixing Yan , Brian Shutes , Gary Bañuelos , Huiyang Wen

The inhibition of salt stress on plant and microbial functions has led to the reduction of nitrogen removal capacity of constructed wetlands (CWs) under saline conditions. The mechanisms and effectiveness of bioaugmented CW (Bio-CW) microcosms with a salt-tolerant microbial inoculum were evaluated for nitrogen removal at different salinity levels. The results showed that the denitrification capacity of CWs was improved under saline conditions by adding the salt-tolerant microbial inoculum. At an EC of 15 mS/cm, the removal percentages of ammonia nitrogen (NH4+-N) and total nitrogen (TN) in Bio-CW microcosms (95.7% and 99.4%) on Day 5 were significantly (p < 0.05) higher than that in unbioaugmented CW (un-Bio-CW) microcosms (68.5% and 76.4%), respectively. The high throughput sequencing data of substrate samples indicated that the microbial community in the CWs was changed by the addition of the salt-tolerant microbial inoculum and the frequency of bacteria with nitrogen removal function was increased in the CWs. Furthermore, both growth and the TN accumulation capacity of plants in Bio-CW microcosms were promoted compared with the un-Bio-CW microcosms. In conclusion, the addition of the salt-tolerant microbial inoculum can enhance the nitrogen removal efficiency of CWs under saline condition via boosting the function of both microorganisms and plants.



中文翻译:

生物强化人工湿地,用于盐水的反硝化:对微生物和植物都有促进作用

盐胁迫对植物和微生物功能的抑制导致在盐分条件下人工湿地(CW)的脱氮能力降低。评价了在不同盐度水平下生物强化的CW(Bio-CW)微观世界与耐盐微生物接种物的机理和有效性。结果表明,通过添加耐盐微生物接种物,可在盐水条件下提高连续水的反硝化能力。在EC为15 mS / cm的情况下,第5天Bio-CW微观世界中氨氮(NH 4 + -N)和总氮(TN)的去除率显着(p。 <0.05)分别高于未增强的CW(un-Bio-CW)微观世界的(68.5%和76.4%)。底物样品的高通量测序数据表明,通过添加耐盐微生物接种物,改变了化学废物中微生物的群落,并且增加了具有脱氮功能的细菌的频率。此外,与非Bio-CW微观世界相比,Bio-CW微观世界中植物的生长和TN积累能力均得到提高。总之,添加耐盐微生物接种物可以通过增强微生物和植物的功能来提高盐水在盐水中的除氮效率。

更新日期:2020-03-09
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