Abstract Vineyards in the New England region of the USA were surveyed for the occurrence of grapevine viruses. A total of ten vineyards were visited and 62 composite samples of leaves with the petioles were collected from symptomatic grapevines (Vitis spp.). All of the samples were assayed by double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) using antibodies specific for four major grapevine leafroll-associated viruses (GLRaV-1, GLRaV-2, GLRaV-3, and GLRaV-4), grapevine fanleaf virus (GFLV), tobacco ringspot virus (TRSV), and tomato ringspot virus (ToRSV). Positive ELISA samples were further tested by reverse transcription polymerase chain reaction (RT-PCR) with primers specific for each of the viruses to confirm the ELISA results. Twenty-two samples were infected with at least one of the viruses tested. GLRaV-3 (24.19%) was the most prevalent virus detected followed by GLRaV-1 (12.90%), ToRSV (3.23%), and GLRaV-2 and TRSV (1.61%). This is the first study reporting on the presence of grapevine viruses in New England. Extensive surveys need to be conducted to evaluate the prevalence and economic impact of these viruses on New England vineyards.

中文翻译：

---

在新英格兰葡萄园中检测到多种葡萄病毒

摘要 对美国新英格兰地区的葡萄园进行了葡萄病毒的调查。总共访问了 10 个葡萄园，从有症状的葡萄藤（Vitis spp.）中收集了 62 个带叶柄的叶子复合样本。所有样品均通过双抗体夹心酶联免疫吸附测定 (DAS-ELISA) 进行测定，使用四种主要葡萄卷叶相关病毒（GLRaV-1、GLRaV-2、GLRaV-3 和 GLRaV-4）的特异性抗体、葡萄扇叶病毒 (GFLV)、烟草环斑病毒 (TRSV) 和番茄环斑病毒 (ToRSV)。通过逆转录聚合酶链反应 (RT-PCR) 使用对每种病毒具有特异性的引物进一步测试阳性 ELISA 样品，以确认 ELISA 结果。22 个样本感染了至少一种所测试的病毒。GLRaV-3 (24. 19%) 是检测到的最流行的病毒，其次是 GLRaV-1 (12.90%)、ToRSV (3.23%)、GLRaV-2 和 TRSV (1.61%)。这是第一项关于新英格兰存在葡萄藤病毒的研究。需要进行广泛的调查来评估这些病毒对新英格兰葡萄园的流行和经济影响。