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The influence of MoO3-NPs on agro-morphological criteria, genomic stability of DNA, biochemical assay, and production of common dry bean (Phaseolus vulgaris L.).
Plant Physiology and Biochemistry ( IF 6.1 ) Pub Date : 2020-03-09 , DOI: 10.1016/j.plaphy.2020.03.009
Samira A Osman 1 , Dina M Salama 2 , M E Abd El-Aziz 3 , Essam A Shaaban 4 , Mohamed S Abd Elwahed 5
Affiliation  

Molybdenum is considered one of the most important micronutrients applied as a foliar fertilizer for common dry bean. In this study, molybdenum oxide nanoparticles (MoO3-NPs) were applied in different concentrations (0, 10, 20, 30 and 40 ppm) over two sequent seasons, 2018 and 2019, to investigate their effect on the plant morphological criteria, yield, and the genomic stability of DNA. The results showed that the application of 40 ppm MoO3-NPs as a foliar fertilizer showed preferable values of plant morphological criteria, such as the number of leaves and branches per plant, as well as the fresh and dry weight with regard to the common bean plant. In addition, the seed yield increased by 82.4% and 84.1% with 40 ppm, while the shoot residue increased by 32.2% and 32.1% with 20 ppm of MoO3-NPs during two seasons, 2018 and 2019, respectively. Furthermore, the common bean treated with 20 and 40 ppm MoO3-NPs had positive unique bands with ISSR primer 848 at 1400 bp (Rf 0.519) and with primer ISSR2M at 200 bp (Rf 0.729), respectively. In addition, SDS-PAGE reveald some proteins in seedlings which were absent in the flowering stage at 154, 102, 64, 37 and 34 KDa, which may be due to differences in plant proteins required for metabolic processes in each stage. In conclusion, the application of 40 ppm MoO3-NPs was more effective on the productivity of the common bean plants.

中文翻译:

MoO3-NPs对农业形态标准,DNA的基因组稳定性,生化分析和普通干豆(菜豆)的影响。

钼被认为是普通干豆叶面肥料中最重要的微量元素之一。在这项研究中,在2018年和2019年的两个后续季节中以不同的浓度(0、10、20、30和40 ppm)施用了氧化钼纳米颗粒(MoO3-NPs),以研究其对植物形态学标准,产量,以及DNA的基因组稳定性。结果表明,施用40 ppm MoO3-NP作为叶面肥显示了植物形态学标准的优选值,例如每株植物的枝叶数以及相对于普通豆类植物的鲜重和干重。此外,在2018年和2019年两个季节中,MoO3-NPs的种子产量分别为40 ppm和8ppm,分别增加了82.4%和84.1%,而枝条残留量增加了32.2%和32.1%。此外,用20和40 ppm MoO3-NP处理的普通豆在1400 bp处的ISSR引物848(Rf 0.519)和200 bp处的ISSR2M(Rf 0.729)分别具有正的独特条带。此外,SDS-PAGE揭示了在开花期以154、102、64、37和34 KDa的幼苗中缺少的某些蛋白质,这可能是由于每个阶段的代谢过程所需的植物蛋白质不同所致。总之,施用40 ppm MoO3-NPs对普通豆类植物的生产力更为有效。这可能是由于每个阶段的代谢过程所需的植物蛋白差异所致。总之,施用40 ppm MoO3-NPs对普通豆类植物的生产力更为有效。这可能是由于每个阶段的代谢过程所需的植物蛋白差异所致。总之,施用40 ppm MoO3-NPs对普通豆类植物的生产力更为有效。
更新日期:2020-03-09
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