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Response of axillary bud development in garlic (Allium sativum L.) to seed cloves soaked in gibberellic acid (GA3) solution
Journal of Integrative Agriculture ( IF 4.6 ) Pub Date : 2020-03-09 , DOI: 10.1016/s2095-3119(20)63156-2
Hong-jiu LIU , Cai-ping HUANG , Pei-jiang TONG , Xue YANG , Ming-ming CUI , Zhi-hui CHENG

Gibberellins (GAs) are important phytohormones that regulate many developmental processes in plants. Clove, as the reproductive organ of garlic, dramatically affected garlic bulb development. Considering the potential of gibberellins in plant development and our previous studies, we investigated the effect of soaking two types of seed cloves (seed clove-I: without root/shoot sprouting; seed clove-II: with root/shoot sprouting) in GA3 solution on axillary bud development and examined the effect of soaking seed cloves in GA3 solution on bulb development, phytohormone level and sugar content in this study. Results indicated seed clove types, soaking liquids and their interaction significantly affected the number of cloves per bulb and the rate of single-clove bulb. Moreover, soaking seed cloves in 1 mmol L−1 GA3 solution for 24 h not only promoted axillary bud formation and secondary plant growth (equal to tillering or branching), but also slightly increased the number of cloves per bulb and changed bulb structure with a low yield and marketable quality. On the 40th day after GA3 treatment (at axillary bud outgrowth stage), zeatin riboside (ZR) and soluble protein in stem were sharply increased with the increase of GA3, sucrose, fructose and soluble protein in leaf. However, GA3, indole-3-acetic acid (IAA), soluble sugar and sucrose in stem (3.52 ng g−1 fresh weight (FW), 19.88 ng g−1 FW, 237.3 mg g−1 FW, and 8.24 mg g−1 FW, respectively) were significantly decreased on the 40th day after GA3 treatment, compared to the control of water treatment (5.56 ng g−1 FW, 32.96 ng g−1 FW, 263.6 mg g−1 FW, and 10.37 mg g−1 FW, respectively). To our knowledge, these novel results indicate seed cloves soaked in GA3 solution promotes axillary bud formation and outgrowth that caused the changes in plant architecture and bulb structure. Meanwhile, our findings suggest that the level of endogenous plant hormone (GA3, IAA and ZR) cooperates with the content of sugar (sucrose and fructose) in leaf and stem to regulate axillary bud outgrowth in garlic.



中文翻译:

大蒜(Allium sativum L.)的腋芽发育对赤霉素(GA 3)溶液浸种的种子的响应。

赤霉素(GAs)是重要的植物激素,可调节植物的许多发育过程。丁香作为大蒜的生殖器官,极大地影响了大蒜鳞茎的发育。考虑到赤霉素在植物发育中的潜力以及我们先前的研究,我们研究了在GA 3中浸泡两种类型的丁香的效果(种子丁香I:无根/芽萌芽;种子丁香II:有根/芽萌芽)。液对腋芽发育的影响,并研究了GA 3中浸种丁香的效果本研究中关于鳞茎发育,植物激素水平和糖含量的解决方案。结果表明,丁香种子的类型,浸泡液及其相互作用显着影响每个鳞茎的丁香数量和单丁香鳞茎的发生率。此外,将种子丁香在1 mmol L -1 GA 3溶液中浸泡24小时,不仅促进了腋芽的形成和次生植物的生长(等于分er或分枝),而且略微增加了每个鳞茎的丁香数量并改变了鳞茎的结构。低产量和适销对路的质量。GA 3处理后第40天(腋芽生长阶段),随着GA 3的增加,玉米淀粉核苷(ZR)和茎中可溶性蛋白急剧增加。,蔗糖,果糖和叶片中的可溶性蛋白质。但是,茎中的GA 3,吲哚-3-乙酸(IAA),可溶性糖和蔗糖(3.52 ng g -1新鲜重量(FW),19.88 ng g -1 FW,237.3 mg g -1 FW和8.24 mg与水处理的对照(5.56 ng g -1 FW,32.96 ng g -1 FW,263.6 mg g -1 FW和10.37)相比,GA 3处理后第40天的g -1 FW分别显着降低。分别为mg g -1 FW)。就我们所知,这些新颖的结果表明丁香浸入了GA 3解决方案促进腋芽的形成和生长,从而引起植物结构和鳞茎结构的变化。同时,我们的研究结果表明,内源植物激素(GA 3,IAA和ZR)的水平与叶和茎中糖(蔗糖和果糖)的含量协同作用,从而调节了大蒜的腋芽生长。

更新日期:2020-03-09
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