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Screening and degradation characteristics of a tylosin-degrading strain
Journal of Integrative Agriculture ( IF 4.6 ) Pub Date : 2020-03-09 , DOI: 10.1016/s2095-3119(19)62764-4
Chang-qing FENG , Deng-miao CHENG , Yao FENG , Wei-ning QI , Zhen-hu JIA , Louise WEAVER , Yuan-wang LIU , Zhao-jun LI

Antibiotics residues have been accumulating in the environment day by day due to overuse of antibiotics. Recalcitrant antibiotic residues, such as tylosin (TYL), can cause serious environmental problems, which makes it important to eliminate TYL from the environment. It is important to eliminate TYL from the environment. In this study, a strain was isolated and purified from fermentation by-product that came from a TYL production factory. The TYL degrading strain was identified by its morphology, physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16S rDNA-coding genes. The temperature, shaking speed, initial TYL concentration, pH and inoculum sizes were investigated under simulated conditions by using single factor tests. The results showed that TYL2, a high efficient strain was isolated and was identified as Brevibacillus borstelensis. The degradation rate of TYL by this strain could reach to 75% with an initial concentration of 25 mg L−1 within 7 days under conditions of 7% B. borstelensis (v/v, 2×108 CFU mL−1) at pH 7.0 and at 35°C. It is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65% within 7 days. This result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.



中文翻译:

泰乐菌素降解菌的筛选及降解特性

由于过度使用抗生素,抗生素残留物在环境中日益累积。顽强的抗生素残留物,例如泰乐菌素(TYL),会引起严重的环境问题,因此从环境中消除TYL非常重要。从环境中消除TYL非常重要。在这项研究中,从TYL生产工厂的发酵副产物中分离和纯化了一种菌株。通过其形态,生理和生化反应,并对16S rDNA编码基因的PCR扩增片段进行测序,鉴定了TYL降解菌株。在模拟条件下,通过单因素试验研究了温度,摇动速度,初始TYL浓度,pH和接种量。结果表明,分离到高效菌株TYL2,鉴定为波氏短杆菌。在pH 7%borstelensis(v / v,2×10 8 CFU mL -1)下,该菌株在7天内的初始浓度为25 mg L -1时,该菌株的TYL降解率可达到75% 7.0和35°C。有趣的是,该菌株具有非常强的降解天然污水中TYL的能力,在7天内降解率为65%。这一结果可能有助于TYL的降解,并为其他抗生素的降解提供指导。

更新日期:2020-03-09
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