In this study, an innovative approach is proposed for the integral valorization of all sugars (cellulosic and hemicellulosic) contained in a lignocellulosic residue, as is brewer's spent grain (BSG), through the production of an advanced biofuel such as biobutanol. For this purpose, the whole slurry obtained in the microwave assisted dilute sulfuric acid pretreatment under optimized conditions (147 °C, 2 min and 1.26% H₂SO₄) at a biomass loading as high as 15% (w/v) was enzymatically hydrolyzed without previous solid-liquid separation and the highly concentrated solution of sugars recovered was fermented to butanol by Clostridium beijerinckii after detoxification with activated charcoal. In this way, all sugars (pentoses and hexoses) contained in BSG could be fermented using a single bioreactor, leading to 11 g/L of butanol and 16 g/L of ABE, which correspond with butanol and ABE yields of 0.21 and 0.32 g/g, respectively. The mass balance revealed then an overall yield of 91 kg butanol/t BSG and 138 kg ABE/t BSG.

在这项研究中，提出了一种创新方法，可通过生产高级生物燃料（例如生物丁醇）来对木质纤维素残留物中所含的所有糖（纤维素和半纤维素）以及啤酒厂的废谷物（BSG）进行整体增值。为此，在酶促条件下，在优化的条件下（147°C，2分钟和1.26％H ₂ SO ₄），在微波辅助的稀硫酸预处理中获得的全部浆液都以酶法进行了酶促处理（酶活度为15％（w / v））无需事先固液分离即可水解，回收的糖的高浓缩溶液通过拜氏梭菌发酵为丁醇用活性炭排毒后。这样，可以使用单个生物反应器发酵BSG中包含的所有糖（戊糖和己糖），从而产生11 g / L的丁醇和16 g / L的ABE，这对应于丁醇和ABE的产量分别为0.21和0.32 g / g。质量平衡表明，总产量为91千克丁醇/吨BSG和138千克ABE /吨BSG。