当前位置: X-MOL 学术Mol. Syst. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Translational efficiency across healthy and tumor tissues is proliferation-related.
Molecular Systems Biology ( IF 8.5 ) Pub Date : 2020-03-01 , DOI: 10.15252/msb.20199275
Xavier Hernandez-Alias 1 , Hannah Benisty 1 , Martin H Schaefer 1, 2 , Luis Serrano 1, 3, 4
Affiliation  

Different tissues express genes with particular codon usage and anticodon tRNA repertoires. However, the codon-anticodon co-adaptation in humans is not completely understood, nor is its effect on tissue-specific protein levels. Here, we first validated the accuracy of small RNA-seq for tRNA quantification across five human cell lines. We then analyzed the tRNA abundance of more than 8,000 tumor samples from TCGA, together with their paired mRNA-seq and proteomics data, to determine the Supply-to-Demand Adaptation. We thereby elucidate that the dynamic adaptation of the tRNA pool is largely related to the proliferative state across tissues. The distribution of such tRNA pools over the whole cellular translatome affects the subsequent translational efficiency, which functionally determines a condition-specific expression program both in healthy and tumor states. Furthermore, the aberrant translational efficiency of some codons in cancer, exemplified by ProCCA and GlyGGT, is associated with poor patient survival. The regulation of these tRNA profiles is partly explained by the tRNA gene copy numbers and their promoter DNA methylation.

中文翻译:

健康组织和肿瘤组织的转化效率与增殖相关。

不同的组织表达具有特定密码子使用和反密码子 tRNA 库的基因。然而,人类的密码子-反密码子协同适应及其对组织特异性蛋白质水平的影响尚未完全了解。在这里,我们首先验证了小 RNA-seq 在五种人类细胞系中进行 tRNA 定量的准确性。然后,我们分析了 TCGA 中 8,000 多个肿瘤样本的 tRNA 丰度及其配对的 mRNA-seq 和蛋白质组数据,以确定供需适应。因此,我们阐明了 tRNA 库的动态适应很大程度上与跨组织的增殖状态有关。此类 tRNA 库在整个细胞翻译组中的分布会影响随后的翻译效率,从而在功能上决定健康和肿瘤状态下的条件特异性表达程序。此外,癌症中一些密码子的异常翻译效率(例如 ProCCA 和 GlyGGT)与患者生存率低相关。这些 tRNA 谱的调节部分可以通过 tRNA 基因拷贝数及其启动子 DNA 甲基化来解释。
更新日期:2020-03-09
down
wechat
bug