BACKGROUND Acanthamoeba spp. are ubiquitous pathogens which cause granulomatous amoebic encephalitis and disseminated infection. Moreover, Acanthamoeba spp. infection of the cornea leads to Acanthamoeba keratitis. Our previous study showed that the infection of an eyeball may also take place via the migration of trophozoites through the optic nerve from the brain to the eyes. The aim of the study was to analyze the activity of enzymatic antioxidants and the concentration of non-enzymatic antioxidant in the eyes of immunocompetent and immunocompromised mice with disseminated acanthamoebiasis. RESULTS In the immunocompetent mice infected with Acanthamoeba spp. we noted a significant decrease in catalase activity at 8 and 16 days post-infection (dpi). Glutathione reductase activity was significantly lower at 16 dpi compared to the control group and glutathione concentration was statistically higher at 24 dpi than in the control group. In the immunosuppressed mice, a statistically significant increase in glutathione concentration in the eye samples was found at 16 dpi compared to those not infected with Acanthamoeba spp. In the immunosuppressed mice infected with Acanthamoeba spp., glutathione peroxidase activity was statistically lower at 8 dpi, and glutathione concentration was statistically significantly higher at 16 dpi compared to the control group. CONCLUSIONS The inflammatory response in the eyes of hosts with experimental acanthamoebiasis led to changes in the activity of enzymatic antioxidants and the content of non-enzymatic antioxidant. Therefore, the dysregulation of antioxidants may play a role in the pathomechanism of Acanthamoeba eye infection.

中文翻译：

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感染了棘阿米巴属菌的具有免疫能力和免疫抑制的小鼠的眼睛中的抗氧化防御作用。

背景棘阿米巴属菌种。是引起肉芽肿性阿米巴性脑炎和传播感染的普遍病原体。此外，棘阿米巴属。角膜感染会导致棘阿米巴角膜炎。我们以前的研究表明，眼球感染也可能是由于滋养体通过视神经从大脑到眼睛的迁移而发生的。这项研究的目的是分析具有免疫活性和免疫受损的散布棘皮病的小鼠眼睛中的酶抗氧化剂的活性和非酶抗氧化剂的浓度。结果在感染了棘阿米巴菌的免疫活性小鼠中。我们注意到感染后8天和16天（dpi）的过氧化氢酶活性显着下降。与对照组相比，在16 dpi时，谷胱甘肽还原酶活性显着降低，在24 dpi时，谷胱甘肽浓度在统计学上高于对照组。在免疫抑制的小鼠中，与未感染棘阿米巴属菌种的小鼠相比，眼部样品中的谷胱甘肽浓度在16 dpi时具有统计学上的显着增加。与对照组相比，在感染了棘阿米巴菌的免疫抑制小鼠中，谷胱甘肽过氧化物酶活性在8 dpi时统计较低，而在16 dpi时谷胱甘肽浓度在统计学上显着较高。结论实验性棘皮病病宿主眼中的炎症反应导致酶促抗氧化剂的活性和非酶促抗氧化剂的含量发生变化。因此，