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Arg-Glu-Asp-Val Peptide Immobilized on an Acellular Graft Surface Inhibits Platelet Adhesion and Fibrin Clot Deposition in a Peptide Density-Dependent Manner
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-03-09 , DOI: 10.1021/acsbiomaterials.0c00078 Atsushi Mahara 1 , Kentaro Kojima 1, 2 , Yoshiaki Hirano 2 , Tetsuji Yamaoka 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-03-09 , DOI: 10.1021/acsbiomaterials.0c00078 Atsushi Mahara 1 , Kentaro Kojima 1, 2 , Yoshiaki Hirano 2 , Tetsuji Yamaoka 1
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Acellular blood vessels possess high potential to be used as tissue-engineered vascular scaffolds. Previously, a high patency was achieved for an Arg-Glu-Asp-Val (REDV) peptide-immobilized small-diameter acellular graft in a minipig model. Results revealed the potential of the peptide to capture a circulating cell and also to suppress fibrin clot deposition. Here, the effect of REDV peptide density on the blood response under ex vivo blood perfusion conditions was investigated. When endothelial cells or platelets were seeded under static conditions, the number of adherent endothelial cells increased with the increase in peptide density. Platelets scarcely adhered on the surface where the peptide density was above 18.9 × 10–4 molecules per nm3. Fibrin clot deposition and circulating cell capture were evaluated in a minipig extracorporeal circulatory system. The fibrin clot did not form on the peptide-immobilized surface, in the range of peptide modification density that was evaluated, whereas the unmodified surface was covered with microthrombi. REDV-specific blood circulating cells were captured on the peptide-immobilized surface with a density above 18.9 × 10–4 molecules per nm3. These results illustrated, under ex vivo blood perfusion conditions, that the REDV-immobilized acellular surface was able to capture cells and also suppress platelet adhesion and fibrin clot deposition in a peptide density-dependent manner.
中文翻译：
固定在无细胞移植物表面上的Arg-Glu-Asp-Val肽以肽密度依赖的方式抑制血小板粘附和纤维蛋白凝集沉积。
无细胞血管具有用作组织工程血管支架的巨大潜力。以前，在小型猪模型中，固定有Arg-Glu-Asp-Val（REDV）肽的小直径无细胞移植物实现了高通畅性。结果揭示了该肽捕获循环细胞并抑制血纤蛋白凝块沉积的潜力。在这里，研究了REDV肽密度对离体血液灌注条件下血液反应的影响。当在静态条件下接种内皮细胞或血小板时，粘附的内皮细胞的数目随着肽密度的增加而增加。血小板几乎不粘附在肽密度高于18.9×10 –4分子/ nm 3的表面上。在小型猪体外循环系统中评估纤维蛋白凝块沉积和循环细胞捕获。在评估的肽修饰密度范围内，未在固定肽的表面上未形成纤维蛋白凝块，而未修饰的表面被微血栓覆盖。REDV特异的血液循环细胞以18.9×10 –4分子/ nm 3的密度被捕获在固定肽的表面上。这些结果说明，在离体血液灌注条件下，固定化REDV的脱细胞表面能够捕获细胞，并且还能够以肽密度依赖性的方式抑制血小板粘附和纤维蛋白凝块沉积。
更新日期：2020-04-23
中文翻译：
固定在无细胞移植物表面上的Arg-Glu-Asp-Val肽以肽密度依赖的方式抑制血小板粘附和纤维蛋白凝集沉积。
无细胞血管具有用作组织工程血管支架的巨大潜力。以前，在小型猪模型中，固定有Arg-Glu-Asp-Val（REDV）肽的小直径无细胞移植物实现了高通畅性。结果揭示了该肽捕获循环细胞并抑制血纤蛋白凝块沉积的潜力。在这里，研究了REDV肽密度对离体血液灌注条件下血液反应的影响。当在静态条件下接种内皮细胞或血小板时，粘附的内皮细胞的数目随着肽密度的增加而增加。血小板几乎不粘附在肽密度高于18.9×10 –4分子/ nm 3的表面上。在小型猪体外循环系统中评估纤维蛋白凝块沉积和循环细胞捕获。在评估的肽修饰密度范围内，未在固定肽的表面上未形成纤维蛋白凝块，而未修饰的表面被微血栓覆盖。REDV特异的血液循环细胞以18.9×10 –4分子/ nm 3的密度被捕获在固定肽的表面上。这些结果说明，在离体血液灌注条件下，固定化REDV的脱细胞表面能够捕获细胞，并且还能够以肽密度依赖性的方式抑制血小板粘附和纤维蛋白凝块沉积。
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