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Isolation and characterization of a new MATE gene located in the same chromosome arm of the aluminium tolerance (Alt1) rye locus.
Plant Biology ( IF 4.2 ) Pub Date : 2020-03-05 , DOI: 10.1111/plb.13107 E Santos 1 , M Matos 1, 2 , C Benito 3
中文翻译：
位于铝耐受性（Alt1）黑麦基因座同一染色体臂上的新MATE基因的分离和鉴定。
更新日期：2020-03-05
Plant Biology ( IF 4.2 ) Pub Date : 2020-03-05 , DOI: 10.1111/plb.13107 E Santos 1 , M Matos 1, 2 , C Benito 3
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- Aluminium (Al) toxicity is the major constraint for crop productivity in acid soils. Wild rye species (Secale spp.) exhibit high Al tolerance, being a good source of genes related to this trait. The Alt1 locus located on the 6RS chromosome arm is one of the four main loci controlling Al tolerance in rye and is known to harbour major genes but, so far, none have been found.
- Through synteny among the short arm of the rye chromosome 6R and the main grass species, we found a candidate MATE gene for the Atl1 locus, later named ScMATE3 , which was isolated and characterized in different Secale species.
- The sequence comparisons revealed both intraspecific and interspecific variability, with high sequence conservation in the Secale genus. SNP with replacement substitution that changed the structure of the protein and can be involved in the Al tolerance trait were found in ScMATE3 gene. The predicted subcellular localization of ScMATE3 is the vacuolar membrane which, together with the phylogenetic relationships performed with other MATE genes of the Poaceae related to Al detoxification, suggest involvement of ScMATE3 in an internal tolerance mechanism. Moreover, expression studies of this gene in rye corroborate its contribution in some Al resistance mechanisms.
- The ScMATE3 gene is located on the 6RS chromosome arm between the same markers in which the Alt1 locus is involved in Al resistance mechanisms in rye, thus being a good candidate gene for this function.
中文翻译：
位于铝耐受性（Alt1）黑麦基因座同一染色体臂上的新MATE基因的分离和鉴定。
- 铝（Al）毒性是酸性土壤中作物生产力的主要限制因素。野生黑麦种（Secale spp。）表现出很高的铝耐受性，是与该性状相关的基因的良好来源。位于6RS染色体臂上的Alt1基因座是控制黑麦Al耐性的四个主要基因座之一，并且已知具有主要基因，但到目前为止，尚未发现。
- 通过黑麦染色体6R短臂与主要草种的协同作用，我们发现了Atl1基因座的候选MATE基因，其后命名为ScMATE3，该基因已在不同的Secale物种中进行了分离和鉴定。
- 序列比较揭示了种内和种间变异性，Secale属具有高度的序列保守性。在ScMATE3基因中发现了具有替换取代的SNP，其改变了蛋白质的结构并且可以参与A1耐性性状。ScMATE3的预测亚细胞定位是液泡膜，与禾本科其他与铝解毒相关的MATE基因发生的系统发育关系表明，ScMATE3参与了内部耐受机制。此外，该基因在黑麦中的表达研究证实了其在某些抗铝机制中的作用。
- 所述ScMATE3基因位于6RS，其中相同的标记之间的染色体臂ALT1基因座是参与的Al抗性机制在黑麦，因此是用于该功能的良好候选基因。
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