In fluorescence microscopy imaging, the segmentation of adjacent cell membranes within cell aggregates, multicellular samples, tissue, organs, or whole organisms remains a challenging task. The lipid bilayer is a very thin membrane when compared to the wavelength of photons in the visual spectra. Fluorescent molecules or proteins used for labelling membranes provide a limited signal intensity, and light scattering in combination with sample dynamics during in vivo imaging lead to poor or ambivalent signal patterns that hinder precise localisation of the membrane sheets. In the proximity of cells, membranes approach and distance each other. Here, the presence of membrane protrusions such as blebs; filopodia and lamellipodia; microvilli; or membrane vesicle trafficking, lead to a plurality of signal patterns, and the accurate localisation of two adjacent membranes becomes difficult.

中文翻译：

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通过分段活动轮廓优化多细胞聚集体中的相邻膜分割和参数化

在荧光显微镜成像中，细胞聚集体、多细胞样本、组织、器官或整个生物体中相邻细胞膜的分割仍然是一项具有挑战性的任务。与可见光谱中的光子波长相比，脂质双层是非常薄的膜。用于标记膜的荧光分子或蛋白质提供有限的信号强度，并且在体内成像期间光散射与样品动力学相结合导致阻碍膜片精确定位的较差或矛盾的信号模式。在细胞附近，膜彼此接近并远离。在这里，膜突起如气泡的存在；丝状伪足和片状伪足；微绒毛；或膜囊泡运输，导致多种信号模式，