Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Efficiency-enhanced and sidelobe-suppressed super-oscillatory lenses for sub-diffraction-limit fluorescence imaging with ultralong working distance
Nanoscale ( IF 5.8 ) Pub Date : 2020/03/06 , DOI: 10.1039/c9nr10697a Wenli Li 1, 2, 3, 4, 5 , Pei He 1, 2, 3, 4, 5 , Weizheng Yuan 1, 2, 3, 4, 5 , Yiting Yu 1, 2, 3, 4, 5
Nanoscale ( IF 5.8 ) Pub Date : 2020/03/06 , DOI: 10.1039/c9nr10697a Wenli Li 1, 2, 3, 4, 5 , Pei He 1, 2, 3, 4, 5 , Weizheng Yuan 1, 2, 3, 4, 5 , Yiting Yu 1, 2, 3, 4, 5
Affiliation
|
Super-oscillatory lens (SOL) optical microscopy, behaving as a non-invasive and universal imaging technique, as well as being a simple post-processing procedure, may provide a potential application for sub-diffraction-limit fluorescence imaging. However, the low energy concentration, high-intensity sidelobes and micrometer-scale working distance of the reported planar SOLs impose unavoidable restrictions on the ground-state applications. Here, we demonstrate step-shaped SOLs based on the multiple-phase-modulated (MPM) method to improve the focusing efficiency. Two pivotal advantages are thus generated: (i) the fabrication complexity can be effectively reduced based on several conventional optical lithography steps; (ii) the focusing efficiency is much higher than that of the random MPM ones due to the efficient manipulation of the wavefronts, bringing about a stronger light concentration to the focal spot. Additionally, the ratio of the sidelobe intensity is flexibly tuned to meet the customized requirements, and a 2 mm-working-distance MPM SOL with the sidelobe intensity highly suppressed is finally exploited. For the first time, as far as we know, a SOL-based fluorescence microscopy without the pinhole filter to map the horizontal morphology of the dispersive fluorescent particles is established. Compared with the results achieved by the conventional wide-field microscopy, the sample details beating the diffraction limit can be reconstructed by simple imaging fusion. This research demonstrates the promising applications of SOLs for low-cost, simplified and highly customized sub-diffraction-limit fluorescence imaging systems free from photobleaching and an extremely short working distance.
中文翻译：
具有超长工作距离的亚衍射极限荧光成像效率增强和旁瓣抑制的超级振荡透镜
作为一种非侵入性和通用成像技术，以及作为一种简单的后处理程序，超级振荡透镜（SOL）光学显微镜可能为亚衍射极限荧光成像提供潜在的应用。但是，所报道的平面SOL的低能量集中度，高强度旁瓣和微米级工作距离对基态应用施加了不可避免的限制。在这里，我们演示了基于多相调制（MPM）方法的阶梯形SOL，以提高聚焦效率。因此产生了两个关键的优点：（i）基于几个常规的光学光刻步骤，可以有效地降低制造复杂性；（ii）由于波前的有效操纵，聚焦效率远高于随机MPM，使焦点更集中。另外，旁瓣强度的比率可以灵活地调整以满足定制的要求，并且最终开发了具有高度抑制的旁瓣强度的2 mm工作距离MPM SOL。据我们所知，这是第一次建立了不使用针孔滤光片来绘制分散型荧光颗粒的水平形态图的基于SOL的荧光显微镜。与常规宽视野显微镜获得的结果相比，可以通过简单的成像融合来重建超过衍射极限的样品细节。这项研究证明了SOL在低成本，
更新日期：2020-04-03
中文翻译：
具有超长工作距离的亚衍射极限荧光成像效率增强和旁瓣抑制的超级振荡透镜
作为一种非侵入性和通用成像技术，以及作为一种简单的后处理程序，超级振荡透镜（SOL）光学显微镜可能为亚衍射极限荧光成像提供潜在的应用。但是，所报道的平面SOL的低能量集中度，高强度旁瓣和微米级工作距离对基态应用施加了不可避免的限制。在这里，我们演示了基于多相调制（MPM）方法的阶梯形SOL，以提高聚焦效率。因此产生了两个关键的优点：（i）基于几个常规的光学光刻步骤，可以有效地降低制造复杂性；（ii）由于波前的有效操纵，聚焦效率远高于随机MPM，使焦点更集中。另外，旁瓣强度的比率可以灵活地调整以满足定制的要求，并且最终开发了具有高度抑制的旁瓣强度的2 mm工作距离MPM SOL。据我们所知，这是第一次建立了不使用针孔滤光片来绘制分散型荧光颗粒的水平形态图的基于SOL的荧光显微镜。与常规宽视野显微镜获得的结果相比，可以通过简单的成像融合来重建超过衍射极限的样品细节。这项研究证明了SOL在低成本，
京公网安备 11010802027423号