Design of hydroxyapatite bioceramics with micro-/nano-topographies to regulate the osteogenic activities of bone morphogenetic protein-2 and bone marrow stromal cells,Nanoscale

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Design of hydroxyapatite bioceramics with micro-/nano-topographies to regulate the osteogenic activities of bone morphogenetic protein-2 and bone marrow stromal cells
Nanoscale ( IF 5.8 ) Pub Date : 2020/03/06 , DOI: 10.1039/c9nr10561a
Xiangfeng Li _{1,

2,

3,

4} , Minjun Liu _{1,

2,

3,

4} , Fuying Chen _{1,

2,

3,

4} , Yuyi Wang _{1,

2,

3,

4} , Menglu Wang _{1,

2,

3,

4} , Xuening Chen _{1,

2,

3,

4} , Yumei Xiao _{1,

2,

3,

4} , Xingdong Zhang _{1,

2,

3,

4}

Affiliation

Biomimicking the nanostructure of natural bone apatite to enhance the bioactivity of hydroxyapatite (HA) biomaterials is an eternal topic in the bone regeneration field. In the present study, we designed four kinds of HA bioceramics with micro- to nanosized grains and investigated the effects of bioceramic topographies on the structures of bone morphogenetic protein-2 (BMP-2) and the effects on the responses of bone marrow stromal cells (BMSCs). Compared to the samples with submicron-scale crystalline particles, HA bioceramics with grain sizes of 104.6 ± 27.8 nm exhibited increased roughness, improved hydrophilicity and enhanced mechanical properties. The synergistic effects of these surface characteristics could well maintain the conformation of BMP-2, facilitate cell adhesion and spreading, and activate the osteogenic differentiation of BMSCs. Furthermore, SBF immersion and in vivo canine intramuscular implantation confirmed that the HA bioceramics with nanotopography also processed excellent bone-like apatite forming ability and outstanding osteoinductivity. In summary, these findings suggest that the nanotopography of HA bioceramics is a critical factor to enhance their bioactivity and osteoinductivity.

中文翻译：

设计具有微/纳米形貌的羟磷灰石生物陶瓷，以调节骨形态发生蛋白2和骨髓基质细胞的成骨活性

模仿天然骨磷灰石的纳米结构以增强羟基磷灰石（HA）生物材料的生物活性是骨骼再生领域的永恒课题。在本研究中，我们设计了四种具有微米至纳米颗粒的HA生物陶瓷，并研究了生物陶瓷形貌对骨形态发生蛋白2（BMP-2）的结构以及对骨髓基质细胞应答的影响（BMSC）。与具有亚微米级晶体颗粒的样品相比，粒径为104.6±27.8 nm的HA生物陶瓷表现出增加的粗糙度，改善的亲水性和增强的机械性能。这些表面特性的协同效应可以很好地维持BMP-2的构象，促进细胞粘附和扩散，并激活BMSCs的成骨分化。体内犬肌肉内植入证实具有纳米形貌的HA生物陶瓷还具有出色的骨样磷灰石形成能力和出色的骨诱导能力。总之，这些发现表明，HA生物陶瓷的纳米形貌是增强其生物活性和骨诱导能力的关键因素。

更新日期：2020-04-03

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