microRNAs (miRNAs) play an essential role in the regulation of large gene networks involved in plant somatic embryogenesis (SEG), but the regulatory mechanism of SEG mediated by miRNAs remains unclear. We used small RNA (sRNA) and transcriptome sequencing methods to identify and profile conserved miRNAs and target genes in five classic embryogenic cultures of Lilium davidii var. unicolor. A total of 35,450 unigenes and 1,727,322 sRNA sequences were obtained from the high-throughput sequencing data. After sequence alignment, 314 known miRNAs belonging to more than 47 miRNA families, 44 novel miRNAs and 32 miRNA*s were identified in L. davidii var. unicolor. miR159 was the largest miRNA family and exhibited the highest expression, and 711 target genes were predicted as the corresponding targets for 273 known miRNAs. In addition, we identified miRNAs that are differentially expressed during SEG and explored the expression profiles of 12 miRNAs and 8 targets. The corresponding relationships between the lda-miR167-WRKY/BGAL, lda-miR319-PCF, lda-miR395-FtsZ and lda-miR408/lda-miR397-LAC pairs were ultimately validated via 5′ rapid amplification of cDNA ends (5′ RACE). Furthermore, we summarized candidate miRNA sequence information, monitored the dynamic expression profiles of miRNAs and tested the interactions between miRNAs and their targets. The data revealed the complex regulatory roles of miRNAs during SEG in L. davidii var. unicolor, and these findings contribute to the miRNA functional annotation of Liliaceae and help elucidate the miRNA-mediated regulatory network during plant SEG.

中文翻译：

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候选 miRNA 在百合体胚胎发生过程中调节作用的证据。单色

microRNAs (miRNAs) 在参与植物体细胞胚胎发生 (SEG) 的大基因网络的调控中发挥重要作用，但由 miRNAs 介导的 SEG 调控机制仍不清楚。我们使用小 RNA (sRNA) 和转录组测序方法来鉴定和分析五种经典百合变种胚胎发生培养物中的保守 miRNA 和靶基因。单色。从高通量测序数据中总共获得了 35,450 个 unigenes 和 1,727,322 个 sRNA 序列。序列比对后，在 L. davidii var. 中鉴定了属于超过 47 个 miRNA 家族的 314 个已知 miRNA、44 个新 miRNA 和 32 个 miRNA*。单色。miR159是最大的miRNA家族，表达量最高，711个靶基因被预测为273个已知miRNA的相应靶标。此外，我们鉴定了在 SEG 期间差异表达的 miRNA，并探索了 12 种 miRNA 和 8 个靶标的表达谱。lda-miR167-WRKY/BGAL、lda-miR319-PCF、lda-miR395-FtsZ和lda-miR408/lda-miR397-LAC对之间的对应关系最终通过cDNA末端的5'快速扩增（5' RACE ）。此外，我们总结了候选 miRNA 序列信息，监测了 miRNA 的动态表达谱并测试了 miRNA 与其靶标之间的相互作用。数据揭示了 miRNA 在 L. davidii var. SEG 过程中的复杂调控作用。unicolor，这些发现有助于百合科的 miRNA 功能注释，并有助于阐明植物 SEG 过程中 miRNA 介导的调控网络。