Recent studies have shown that the MdDMR6 is a key gene for the control of columnar growing habit. However, there has been no research report on the function of the MdDMR6 gene. In the present study, the columnar apple shoot cultures exhibited better tolerance than that of the standard apple shoot cultures under the salt stress. The expression of MdDMR6 was remarkably induced by the salt stress. Subsequently, the MdDMR6 was transferred into tobacco and apple calli via the Agrobacterium-mediated approach. Functional analysis showed that the overexpressing transgenic tobacco and apple calli displayed salt tolerance and ABA-insensitive phenotype through physiological and biochemical analysis. The MdDMR6 overexpression activated the transcript levels of several genes involved in the ABA-dependent stress genes under the salt condition. These results suggest that the MdDMR6 would increase the plant tolerance to the salt stress via activating or interacting other protein to indirectly activate ABA-dependent pathways.

中文翻译：

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苹果柱状基因 MdDMR6 提高了转基因烟草幼苗和苹果愈伤组织的盐胁迫耐受性

最近的研究表明，MdDMR6 是控制柱状生长习性的关键基因。但目前还没有关于MdDMR6基因功能的研究报道。在本研究中，柱状苹果芽培养物在盐胁迫下表现出比标准苹果芽培养物更好的耐受性。MdDMR6的表达受盐胁迫显着诱导。随后，通过农杆菌介导的方法将 MdDMR6 转移到烟草和苹果愈伤组织中。功能分析表明，过表达的转基因烟草和苹果愈伤组织通过生理生化分析显示出耐盐性和对ABA不敏感的表型。MdDMR6 过表达激活了盐条件下参与 ABA 依赖性应激基因的几个基因的转录水平。