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EST-SSRs reveal genetic distinction between lac and grain yielding genotypes of pigeonpea
Journal of Plant Biochemistry and Biotechnology ( IF 1.9 ) Pub Date : 2020-03-04 , DOI: 10.1007/s13562-020-00558-9
Thamilarasi Kandasamy , Kanchan Kumari , Jyotirmoy Ghosh , Kishor U. Tribhuvan , Vaibhav D. Lohot , Madhuranjana Gargi , S. Ghosal

Pigeonpea, an important legume crop is a good host plant for lac cultivation in North East India. In the present study, sixty-three polymorphic EST microsatellite markers were screened across the twenty-seven pigeonpea genotypes collected from different parts of India. The markers used in the study amplified a total of 352 alleles with an average high polymorphic information content of 0.75. The number of alleles per locus varied from 1 to 16 with an average of 5.58, indicating a greater magnitude of diversity among the genotypes. From the Nei’s analysis it was found that maximum diversity exists between the genotypes RCMP 3 and Pant Arhar-291. AMOVA showed a significant amount of variability (63.37%) among the genotypes compared to individuals within the groups or genotypes (36.63%). Mean Shannon information index was 1.15 demonstrating high rate of genetic variability. The neutrality of each marker tested by Ewens–Watterson test for neutrality suggested that all the microsatellite loci except ASSR-70, ASSR-228, ASSR-230 and ASSR-1432 were neutral. According to the dendrogram based on Nei′s analysis the whole population was divided into two major clusters. The first cluster comprised of sixteen local germplasm responsible for high broodlac yield and the second cluster contained eleven high grain yielding varieties. Molecular markers can be effectively used for the selection of elite germplasm of pigeonpea for lac cultivation and high yield of pulses, and consequently benefit farmers and consumers of this important pulse crop of India and the semi-arid regions of the world.

中文翻译:

EST-SSRs揭示了木豆的lac和籽粒产量基因型之间的遗传差异

木豆是重要的豆类作物,是印度东北部紫胶种植的良好寄主植物。在本研究中,从印度不同地区收集的27种木豆基因型中筛选了63种多态EST微卫星标记。研究中使用的标记扩增了总共352个等位基因,平均高多态信息含量为0.75。每个基因座的等位基因数量从1到16不等,平均为5.58,表明基因型之间的多样性更大。根据Nei的分析,发现在RCMP 3和Pant Arhar-291基因型之间存在最大多样性。与各组或基因型内的个体(36.63%)相比,AMOVA在基因型间显示出显着的变异性(63.37%)。平均Shannon信息指数为1。15表明遗传变异率很高。通过Ewens–Watterson检验测试的每种标记物的中性均表明,除ASSR-70,ASSR-228,ASSR-230和ASSR-1432以外的所有微卫星基因座都是中性的。根据基于Nei分析的树状图,整个人口被分为两个主要群体。第一组由十六种本地种质组成,可带来高的溴丁酸产量,第二组包含十一种高谷物生产品种。分子标记可以有效地用于选择紫胶栽培的木豆种子优良种质和豆类的高产,因此使印度和世界半干旱地区这种重要的豆类作物的农民和消费者受益。通过Ewens-Watterson检验测试的每种标记物的中性表明,除ASSR-70,ASSR-228,ASSR-230和ASSR-1432以外的所有微卫星基因座都是中性的。根据基于Nei分析的树状图,整个人口被分为两个主要群体。第一组由十六种本地种质组成,可带来高的溴丁酸产量,第二组包含十一种高谷物生产品种。分子标记可以有效地用于选择紫胶栽培的木豆树种质和豆类的高产,因此使印度和世界半干旱地区这种重要的豆类作物的农民和消费者受益。通过Ewens-Watterson检验测试的每种标记物的中性表明,除ASSR-70,ASSR-228,ASSR-230和ASSR-1432以外的所有微卫星基因座都是中性的。根据基于Nei分析的树状图,整个人口被分为两个主要群体。第一组由十六种本地种质组成,可带来高的溴丁酸产量,第二组包含十一种高产谷物品种。分子标记可以有效地用于选择紫胶栽培的木豆树种质和豆类的高产,因此使印度和世界半干旱地区这种重要的豆类作物的农民和消费者受益。根据基于Nei分析的树状图,整个人口被分为两个主要群体。第一组由十六种本地种质组成,可带来高的溴丁酸产量,第二组包含十一种高产谷物品种。分子标记可以有效地用于选择紫胶栽培的木豆树种质和豆类的高产,因此使印度和世界半干旱地区这种重要的豆类作物的农民和消费者受益。根据基于Nei分析的树状图,整个人口被分为两个主要群体。第一组由十六种本地种质组成,可带来高的溴丁酸产量,第二组包含十一种高产谷物品种。分子标记可以有效地用于选择紫胶栽培的木豆种子优良种质和豆类的高产,因此使印度和世界半干旱地区这种重要的豆类作物的农民和消费者受益。
更新日期:2020-03-04
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