To validate the feasibility of two types of bioactive glass that contains spherical and radical spherical nano-sized particles in promoting bone repair, we hypothesize that radical spherical nano-sized particles have higher bone repair effectiveness than spherical one due to the physicochemical properties. We rigorously compared the physicochemical properties and bioactivities of these two types of bioactive glass. Specifically, we measured the size, surface morphology, concentration of ionic-dissolution products, bioactivity, and biological effects of two groups of bioactive glass on rat bone marrow mesenchymal stem cells (rBMSCs) and evaluate their effect on proliferation and osteogenic differentiation of rBMSCs in vitro. We observed that spherical nano-bioactive glass (SNBG) was spherical with smooth boundary, while the radial spherical nano-bioactive glass (RSNBG) had radial pore on the surface of particle boundary. When the two materials were immersed in simulated body fluid for 24 h, RSNBG produced more and denser hydroxyapatite carbonate than SNBG. The concentration of Ca and Si ions in RSNBG 24 h extract is higher than that of SNBG, while the concentration of P ions is lower. Proliferation, alkaline phosphatase (ALP) activity, intracellular Ca ion concentrations defined as the number of mineralized nodules produced, and the expression of osteogenic genes were significantly higher in rBMSCs co-cultured with 50 µg/mL RSNBG than SNBG. Overall, these results validated our hypothesis that RSNBG can provide better benefit than SNBG for inducing proliferation and osteogenic differentiation in rBMSCs, in turn suggested the feasibility of this RSNBG in further studies and utilization toward the ends of improved bone repair effectiveness.

为了验证包含球形和自由基球形纳米尺寸颗粒的两种类型的生物活性玻璃在促进骨修复中的可行性，我们假设，由于物理化学性质，自由基球形纳米尺寸颗粒具有比球形的更高的骨修复功效。我们严格比较了这两种类型的生物活性玻璃的理化性质和生物活性。具体来说，我们测量了两组生物活性玻璃对大鼠骨髓间充质干细胞（rBMSCs）的大小，表面形态，离子溶解产物的浓度，生物活性和生物学效应，并评估了它们对rBMSC增殖和成骨分化的影响。体外。我们观察到球形纳米生物活性玻璃（SNBG）是具有光滑边界的球形，径向球形纳米生物活性玻璃（RSNBG）在颗粒边界表面具有径向孔。当将两种材料浸入模拟体液中24 h时，RSNBG比SNBG产生更多，更致密的羟基磷灰石碳酸盐。RSNBG 24 h提取物中Ca和Si离子的浓度高于SNBG，而P离子的浓度较低。在50μg/ mL RSNBG中共培养的rBMSC中，增殖，碱性磷酸酶（ALP）活性，细胞内Ca离子浓度（定义为产生的矿化结节的数量）和成骨基因的表达明显高于SNBG。总体而言，这些结果证实了我们的假设，即RSNBG在诱导rBMSC增殖和成骨分化方面比SNBG更好，