Key message

Two QTLs on ChrB09 significantly associated with both early and late leaf spots were identified by genome-wide association study in the US peanut mini-core collection.

Abstract

Early leaf spot (ELS) and late leaf spot (LLS) are two serious peanut diseases in the USA, causing tens of millions of dollars of annual economic losses. However, the genetic factors underlying resistance to those diseases in peanuts have not been well-studied. We conducted a genome-wide association study for the two peanut diseases using Affymetrix version 2.0 SNP array with 120 genotypes mainly coming from the US peanut mini-core collection. A total of 46 quantitative trait loci (QTLs) were identified with phenotypic variation explained (PVE) from 10.19 to 24.11%, in which eighteen QTLs are for resistance to ELS and 28 QTLs for LLS. Among the 46 QTLs, there were four and two major QTLs with PVE higher than 16.99% for resistance ELS and LLS, respectively. Of the six major QTLs, five were located on the B sub-genome and only one was on the A sub-genome, which suggested that the B sub-genome has more potential resistance genomic regions than the A sub-genome. In addition, two genomic regions on chromosome B09 were found to provide significant resistance to both ELS and LLS. A total of 74 non-redundant genes were identified as resistance genes, among which, twelve candidate genes were in significant genomic regions including two candidate genes for both ELS and LLS, and other ten candidate genes for ELS. The QTLs and candidate genes obtained from this study will be useful to breed peanuts for resistances to the diseases.

中文翻译：

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通过GWAS分析鉴定栽培花生（Arachis hypogaea L.）叶斑病抗性QTL。

关键信息

在美国花生微型核心收集中，通过全基因组关联研究确定了 ChrB09 上与早期和晚期叶斑显着相关的两个 QTL。

抽象的

早期叶斑病（ELS）和晚期叶斑病（LLS）是美国两种严重的花生病害，每年造成数千万美元的经济损失。然而，花生抵抗这些疾病的遗传因素尚未得到充分研究。我们使用 Affymetrix 2.0 版 SNP 阵列对这两种花生疾病进行了全基因组关联研究，其中 120 种基因型主要来自美国花生迷你核心收集。共鉴定出46个数量性状位点（QTL），表型变异解释（PVE）从10.19%到24.11%，其中18个QTL用于ELS抗性，28个QTL用于LLS。46个QTL中，抗性ELS和LLS分别有4个和2个PVE高于16.99%的主要QTL。六大QTL中，5个位于B亚基因组上，只有1个位于A亚基因组上，这表明B亚基因组比A亚基因组具有更多潜在的抗性基因组区域。此外，发现染色体 B09 上的两个基因组区域对 ELS 和 LLS 都具有显着的抗性。共有74个非冗余基因被鉴定为抗性基因，其中12个候选基因位于重要的基因组区域，其中ELS和LLS候选基因2个，ELS候选基因10个。从该研究中获得的QTL和候选基因将有助于培育抗病花生。发现染色体 B09 上的两个基因组区域对 ELS 和 LLS 都具有显着的抗性。共有74个非冗余基因被鉴定为抗性基因，其中12个候选基因位于重要的基因组区域，其中ELS和LLS候选基因2个，ELS候选基因10个。从该研究中获得的QTL和候选基因将有助于培育抗病花生。发现染色体 B09 上的两个基因组区域对 ELS 和 LLS 都具有显着的抗性。共有74个非冗余基因被鉴定为抗性基因，其中12个候选基因位于重要的基因组区域，其中ELS和LLS候选基因2个，ELS候选基因10个。从该研究中获得的QTL和候选基因将有助于培育抗病花生。