Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is the predominant cause of severe enteropathogenic diarrhea in swine. A simple, rapid, specific, and sensitive method is critical for monitoring PEDV on pig farms. In this study, a simple and rapid lateral flow immunoassay detection system that integrates europium (Eu) (III) chelate microparticles was developed to identify PEDV in fecal swabs. This newly developed diagnostic sandwich immunoassay utilizes lateral flow test strips (LFTSs). The fluorescence peak heights of the test line (HT) and the control line (HC) were measured using a fluorescence strip reader, and the HT/HC ratio was used for quantitation. The limit of detection of PEDV with this LFTS was ??ten times the median tissue culture infectious dose (TCID50) per mL??. Fecal swab samples were used to determine the cutoff value. Field samples, various PEDV strains and other viruses were used to determine the sensitivity and specificity of the Eu (III) chelate microparticle-based LFTSs, which were 97.8% and 100%, respectively, with a cutoff value of 0.05, as compared with reverse transcription polymerase chain reaction (RT-PCR). In samples from piglets experimentally infected with PEDV, the results were in high agreement with those obtained by RT-PCR. Epidemiological surveillance of PEDV using the LFTSs ??in areas threatened by African swine fever virus?? suggested that the PEDV positive rate on pig farms had significantly decreased, mainly due to the implementation of strict biosecurity measures. The results indicate that the Eu (III) chelate microparticle-based LFTS system is a rapid, sensitive, and reliable method for the identification of PEDV, indicating its suitability for epidemiological surveillance of PEDV infection.

中文翻译：

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快速，灵敏的基于che（III）螯合物微粒的侧向流动测试条的开发，用于检测和流行病学监测猪流行性腹泻病毒。

猪流行性腹泻病毒（PEDV）是Alphacoronavirus属的一种，是猪严重肠病性腹泻的主要原因。一种简单，快速，特异且敏感的方法对于监控养猪场的PEDV至关重要。在这项研究中，开发了一种简单而快速的侧流免疫分析检测系统，该系统整合了（（Eu）（III）螯合物微粒，以鉴定粪便拭子中的PEDV。这种新开发的诊断性三明治免疫测定法利用侧向流动测试条（LFTS）。使用荧光条读取器测量测试线（HT）和对照线（HC）的荧光峰高，并将HT / HC比用于定量。用这种LFTS检测PEDV的极限是每毫升组织培养物感染中位数（TCID50）的十倍。粪便拭子样本用于确定临界值。使用现场样品，各种PEDV毒株和其他病毒来确定基于Eu（III）螯合物微粒的LFTS的敏感性和特异性，分别为97.8％和100％，截断值为0.05，反之则为0.05。转录聚合酶链反应（RT-PCR）。在用PEDV实验感染的仔猪样本中，结果与RT-PCR的结果高度吻合。在非洲猪瘟病毒威胁地区使用LFTS对PEDV进行流行病学监测 提示养猪场的PEDV阳性率已大大降低，这主要是由于实施了严格的生物安全措施。结果表明，基于Eu（III）螯合物微粒的LFTS系统是快速，灵敏，