N-Terminal Acetylation Stabilizes SIGMA FACTOR BINDING PROTEIN1 Involved in Salicylic Acid-Primed Cell Death.,Plant Physiology

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N-Terminal Acetylation Stabilizes SIGMA FACTOR BINDING PROTEIN1 Involved in Salicylic Acid-Primed Cell Death.
Plant Physiology ( IF 6.5 ) Pub Date : 2020-03-05 , DOI: 10.1104/pp.19.01417
Zihao Li _{1,

2} , Vivek Dogra ₁ , Keun Pyo Lee ₁ , Rongxia Li ₁ , Mingyue Li _{1,

2} , Mengping Li _{1,

2} , Chanhong Kim ₃

Affiliation

N-terminal (Nt) acetylation (NTA) is an ample and irreversible cotranslational protein modification catalyzed by ribosome-associated Nt-acetyltransferases. NTA on specific proteins can act as a degradation signal (called an Ac/N-degron) for proteolysis in yeast and mammals. However, in plants, the biological relevance of NTA remains largely unexplored. In this study, we reveal that Arabidopsis (Arabidopsis thaliana) SIGMA FACTOR-BINDING PROTEIN1 (SIB1), a transcription coregulator and a positive regulator of salicylic acid-primed cell death, undergoes an absolute NTA on the initiator Met; Nt-acetyltransferase B (NatB) partly contributes to this modification. While NTA results in destabilization of certain target proteins, our genetic and biochemical analyses revealed that plant NatB-involved NTA instead renders SIB1 more stable. Given that the ubiquitin/proteasome system stimulates SIB1 degradation, it seems that the NTA-conferred stability ensures the timely expression of SIB1-dependent genes, mostly related to immune responses. Taking our findings together, here we report a noncanonical NTA-driven protein stabilization in land plants.

中文翻译：

N末端乙酰化可稳定参与水杨酸引发的细胞死亡的SIGMA因子结合蛋白1。

N末端（Nt）乙酰化（NTA）是核糖体相关Nt-乙酰基转移酶催化的大量且不可逆的共翻译蛋白修饰。特定蛋白质上的NTA可以充当降解信号（称为Ac / N-degron），用于酵母和哺乳动物的蛋白水解。但是，在植物中，NTA的生物学相关性尚未得到充分探索。在这项研究中，我们揭示了拟南芥（Arabidopsis thaliana）SIGMA因子结合蛋白1（SIB1），转录共调节剂和水杨酸引发的细胞死亡的正调节剂，在启动子Met上经历了绝对NTA。Nt-乙酰基转移酶B（NatB）部分有助于这种修饰。尽管NTA导致某些目标蛋白不稳定，但我们的遗传和生化分析表明，植物NatB参与的NTA使得SIB1更稳定。鉴于遍在蛋白/蛋白酶体系统刺激SIB1降解，看来NTA赋予的稳定性确保了SIB1依赖性基因的及时表达，而该基因主要与免疫反应有关。综合我们的发现，在这里我们报告陆地植物中非经典NTA驱动的蛋白质稳定化。

更新日期：2020-05-01

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