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The Calcium Sensor CBL2 and Its Interacting Kinase CIPK6 Are Involved in Plant Sugar Homeostasis via Interacting with Tonoplast Sugar Transporter TST2.
Plant Physiology ( IF 6.5 ) Pub Date : 2020-03-05 , DOI: 10.1104/pp.19.01368
Jinwu Deng 1 , Xiyan Yang 1 , Weinan Sun 1 , Yuhuan Miao 1 , Liangrong He 1, 2 , Xianlong Zhang 3
Affiliation  

Calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK)-mediated calcium signaling has been widely reported to function in plant development and various stress responses, particularly in ion homeostasis. Sugars are the most important primary metabolites, and thus sugar homeostasis requires precise regulation. Here, we describe a CBL2-CIPK6-Tonoplast-Localized Sugar Transporter2 (TST2) molecular module in cotton (Gossypium hirsutum) that regulates plant sugar homeostasis, in particular Glc homeostasis. GhCIPK6 is recruited to the tonoplast by GhCBL2 and interacts with the tonoplast-localized sugar transporter GhTST2. Overexpression of either GhCBL2, GhCIPK6, or GhTST2 was sufficient to promote sugar accumulation in transgenic cotton, whereas RNAi-mediated knockdown of GhCIPK6 expression or CRISPR-Cas9-mediated knockout of GhTST2 resulted in significantly decreased Glc content. Moreover, mutation of GhCBL2 or GhTST2 in GhCIPK6-overexpressing cotton reinstated sugar contents comparable to wild-type plants. Heterologous expression of GhCIPK6 in Arabidopsis (Arabidopsis thaliana) also promoted Glc accumulation, whereas mutation of AtTST1/2 in GhCIPK6-overexpressing Arabidopsis similarly reinstated wild-type sugar contents, thus indicating conservation of CBL2-CIPK6-TST2-mediated sugar homeostasis among different plant species. Our characterization of the molecular players behind plant sugar homeostasis may be exploited to improve sugar contents and abiotic stress resistance in plants.

中文翻译:

钙传感器CBL2及其相互作用的激酶CIPK6通过与Tonoplast糖转运蛋白TST2的相互作用而参与植物糖的体内平衡。

钙调神经磷酸酶B样蛋白(CBL)和CBL相互作用蛋白激酶(CIPK)介导的钙信号已被广泛报道在植物发育和各种胁迫反应中发挥作用,尤其是在离子稳态中。糖是最重要的主要代谢产物,因此糖稳态需要精确调节。在这里,我们描述了棉花(陆地棉)中的CBL2-CIPK6-液泡膜局部糖转运蛋白2(TST2)分子模块,该模块调节植物糖的稳态,特别是Glc的稳态。GhCIPK6被GhCBL2募集到液泡膜中,并与液泡膜定位的糖转运蛋白GhTST2相互作用。GhCBL2,GhCIPK6或GhTST2的过表达足以促进转基因棉花中糖的积累,RNAi介导的GhCIPK6表达敲除或CRISPR-Cas9介导的GhTST2敲除导致Glc含量显着降低。此外,在过表达GhCIPK6的棉花中,GhCBL2或GhTST2的突变恢复了与野生型植物相当的糖含量。GhCIPK6在拟南芥(Arabidopsis thaliana)中的异源表达也促进了Glc的积累,而过表达GhCIPK6的拟南芥中AtTST1 / 2的突变同样恢复了野生型糖含量,因此表明CBL2-CIPK6-TST2介导的糖稳态在不同植物中得以保存。种类。我们对植物糖体内稳态背后的分子作用因子的表征可用于改善植物中的糖含量和非生物逆境抗性。过量表达GhCIPK6的棉花中GhCBL2或GhTST2突变恢复了与野生型植物相当的糖含量。GhCIPK6在拟南芥中的异源表达也促进了Glc的积累,而过表达GhCIPK6的拟南芥中AtTST1 / 2的突变也恢复了野生型糖含量,因此表明CBL2-CIPK6-TST2介导的糖稳态在不同植物中的保守性。种类。我们对植物糖体内稳态背后的分子作用因子的表征可用于改善植物中的糖含量和非生物逆境抗性。过量表达GhCIPK6的棉花中GhCBL2或GhTST2突变恢复了与野生型植物相当的糖含量。GhCIPK6在拟南芥(Arabidopsis thaliana)中的异源表达也促进了Glc的积累,而过表达GhCIPK6的拟南芥中AtTST1 / 2的突变同样恢复了野生型糖含量,因此表明CBL2-CIPK6-TST2介导的糖稳态在不同植物中得以保存。种类。我们对植物糖体内稳态背后的分子作用因子的表征可用于改善植物中的糖含量和非生物逆境抗性。而过表达GhCIPK6的拟南芥中AtTST1 / 2的突变同样恢复了野生型糖含量,从而表明了CBL2-CIPK6-TST2介导的糖稳态在不同植物物种之间的保守性。我们对植物糖体内稳态背后的分子作用因子的表征可用于改善植物中的糖含量和非生物逆境抗性。而过表达GhCIPK6的拟南芥中AtTST1 / 2的突变同样恢复了野生型糖含量,从而表明了CBL2-CIPK6-TST2介导的糖稳态在不同植物物种之间的保守性。我们对植物糖体内稳态背后的分子作用因子的表征可用于改善植物中的糖含量和非生物逆境抗性。
更新日期:2020-05-01
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