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Total glucosides of paeony (TGP) alleviates Sjogren's syndrome through inhibiting inflammatory responses in mice.
Phytomedicine ( IF 6.7 ) Pub Date : 2020-03-06 , DOI: 10.1016/j.phymed.2020.153203 Binbin Li 1 , Ge Liu 1 , Rui Liu 1 , Shucheng He 1 , Xiang Li 1 , Liangliang Huang 1 , Ziyu Wang 1 , Yunman Li 1 , Yongjian Chen 2 , Hong Yin 2 , Weirong Fang 1
Phytomedicine ( IF 6.7 ) Pub Date : 2020-03-06 , DOI: 10.1016/j.phymed.2020.153203 Binbin Li 1 , Ge Liu 1 , Rui Liu 1 , Shucheng He 1 , Xiang Li 1 , Liangliang Huang 1 , Ziyu Wang 1 , Yunman Li 1 , Yongjian Chen 2 , Hong Yin 2 , Weirong Fang 1
Affiliation
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BACKGROUND
Sjogren's syndrome (SS) is an inflammatory autoimmune disease whose etiology is complicated. Total glucosides of paeony (TGP) has a variety of pharmacological effects.
PURPOSE
To evaluate the therapeutic effects of TGP on SS in mice and anti-inflammatory mechanism.
STUDY DESIGN
SS animal model was developed from C57BL/6J mice through immunological induction (SS mice) and NOD/ShiltJNju (NOD) mice. Inflammatory cytokines and other related indicators were measured.
METHODS
TGP (720, 360, 180 mg/kg) was intragastrically administered for 6 or 16 weeks for SS mice and NOD mice, respectively. Average food and water intake, average body weight, saliva flow, submandibular gland (SMG) and spleen index, and SMG pathology were measured. ELISA was used to evaluate serum inflammatory cytokines in SS mice and autoantigens in NOD mice. Real-time PCR, Western blot and Luminex liquid suspension chip assay were applied to analyze SMG inflammatory cytokines mRNA and protein expression of NOD mice.
RESULTS
Compared with SS mice, TGP treatment improved SMG pathological damage. TGP (720 mg/kg) treatment increased saliva flow, and reduced organ indexes and serum IL-6 and IFN-γ concentration. TGP (360 mg/kg) treatment decreased serum IFN-γ concentration. TGP (180 mg/kg) treatment for 6 weeks decreased average body weight. Compared with NOD mice, TGP treatment increased saliva flow from 9 to 15 weeks, decreased body weight, and alleviated pathological damage of SMG after 2 and 16 weeks. After 2 weeks of administration, TGP treatment inhibited serum concentration of SSB/La, SSA/Ro and α-fodrin, decreased TNF-α, IL-1β and IFN-γ in SMG, and down-regulated protein expressions of BAFF and IL-17A and mRNA expressions of BAFF, TNF-α, IL-17A, CXCL9 and CXCL13 in SMG. After 8 weeks of administration, TGP treatment decreased the concentration of α-fodrin in serum, TNF-α and IL-6 in SMG, and down-regulated mRNA expressions of IL-17A, TNF-α, CXCL9, CXCL13 and BAFF and protein expressions of IL-17A and BAFF in SMG. After 16 weeks of administration, TGP treatment reduced serum SSA/Ro, SSB/La and α-fodrin concentration, and decreased BAFF protein expression and TNF-α, CXCL9, CXCL13, IL-17A, and BAFF mRNA expressions.
CONCLUSION
TGP has a certain therapeutic effect on SS mice and NOD mice through inhibiting inflammatory responses.
中文翻译：
eon药总苷（TGP）通过抑制小鼠的炎症反应缓解干燥综合征。
背景技术干燥综合征（SS）是一种炎性自身免疫病，其病因复杂。eon药总苷（TGP）具有多种药理作用。目的评价TGP对小鼠SS的治疗作用及抗炎机制。研究设计SS动物模型是通过C57BL / 6J小鼠通过免疫诱导（SS小鼠）和NOD / ShiltJNju（NOD）小鼠开发的。测量了炎症细胞因子和其他相关指标。方法分别对SS小鼠和NOD小鼠进行TGP（720、360、180 mg / kg）胃内给药6周或16周。测量平均食物和水摄入量，平均体重，唾液流量，下颌下腺（SMG）和脾脏指数以及SMG病理。ELISA用于评估SS小鼠的血清炎性细胞因子和NOD小鼠的自身抗原。采用实时荧光定量PCR，Western blot和Luminex液体悬浮芯片分析法分析NOD小鼠的SMG炎性细胞因子mRNA和蛋白表达。结果与SS小鼠相比，TGP治疗改善了SMG的病理损伤。TGP（720 mg / kg）处理可增加唾液流量，降低器官指数，降低血清IL-6和IFN-γ浓度。TGP（360 mg / kg）处理可降低血清IFN-γ浓度。TGP（180 mg / kg）治疗6周可降低平均体重。与NOD小鼠相比，TGP治疗将唾液流量从9周增加到15周，减轻了体重，并减轻了2周和16周后SMG的病理损伤。给药2周后，TGP治疗抑制了SMG中SSB / La，SSA / Ro和α-fodrin的血药浓度，降低了TNF-α，IL-1β和IFN-γ，SMG中BAFF和IL-17A的蛋白表达和BAFF，TNF-α，IL-17A，CXCL9和CXCL13的mRNA表达下调。给药8周后，TGP治疗降低了血清中α-fodrin的浓度，SMG中的TNF-α和IL-6，并下调了IL-17A，TNF-α，CXCL9，CXCL13和BAFF的mRNA表达和蛋白质IL-17A和BAFF在SMG中的表达 给药16周后，TGP治疗可降低血清SSA / Ro，SSB / La和α-fodrin浓度，并降低BAFF蛋白表达和TNF-α，CXCL9，CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。TGP治疗可降低血清中α-fodrin的浓度，SMG中TNF-α和IL-6的表达，并下调IL-17A，TNF-α，CXCL9，CXCL13和BAFF的mRNA表达以及IL-17A和IL-6的蛋白表达。 SMG中的BAFF。给药16周后，TGP治疗可降低血清SSA / Ro，SSB / La和α-fodrin浓度，并降低BAFF蛋白表达和TNF-α，CXCL9，CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。TGP治疗可降低血清中α-fodrin的浓度，SMG中TNF-α和IL-6的表达，并下调IL-17A，TNF-α，CXCL9，CXCL13和BAFF的mRNA表达以及IL-17A和IL-6的蛋白表达。 SMG中的BAFF。给药16周后，TGP治疗可降低血清SSA / Ro，SSB / La和α-fodrin浓度，并降低BAFF蛋白表达和TNF-α，CXCL9，CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。
更新日期：2020-03-06
中文翻译：
eon药总苷（TGP）通过抑制小鼠的炎症反应缓解干燥综合征。
背景技术干燥综合征（SS）是一种炎性自身免疫病，其病因复杂。eon药总苷（TGP）具有多种药理作用。目的评价TGP对小鼠SS的治疗作用及抗炎机制。研究设计SS动物模型是通过C57BL / 6J小鼠通过免疫诱导（SS小鼠）和NOD / ShiltJNju（NOD）小鼠开发的。测量了炎症细胞因子和其他相关指标。方法分别对SS小鼠和NOD小鼠进行TGP（720、360、180 mg / kg）胃内给药6周或16周。测量平均食物和水摄入量，平均体重，唾液流量，下颌下腺（SMG）和脾脏指数以及SMG病理。ELISA用于评估SS小鼠的血清炎性细胞因子和NOD小鼠的自身抗原。采用实时荧光定量PCR，Western blot和Luminex液体悬浮芯片分析法分析NOD小鼠的SMG炎性细胞因子mRNA和蛋白表达。结果与SS小鼠相比，TGP治疗改善了SMG的病理损伤。TGP（720 mg / kg）处理可增加唾液流量，降低器官指数，降低血清IL-6和IFN-γ浓度。TGP（360 mg / kg）处理可降低血清IFN-γ浓度。TGP（180 mg / kg）治疗6周可降低平均体重。与NOD小鼠相比，TGP治疗将唾液流量从9周增加到15周，减轻了体重，并减轻了2周和16周后SMG的病理损伤。给药2周后，TGP治疗抑制了SMG中SSB / La，SSA / Ro和α-fodrin的血药浓度，降低了TNF-α，IL-1β和IFN-γ，SMG中BAFF和IL-17A的蛋白表达和BAFF，TNF-α，IL-17A，CXCL9和CXCL13的mRNA表达下调。给药8周后，TGP治疗降低了血清中α-fodrin的浓度，SMG中的TNF-α和IL-6，并下调了IL-17A，TNF-α，CXCL9，CXCL13和BAFF的mRNA表达和蛋白质IL-17A和BAFF在SMG中的表达 给药16周后，TGP治疗可降低血清SSA / Ro，SSB / La和α-fodrin浓度，并降低BAFF蛋白表达和TNF-α，CXCL9，CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。TGP治疗可降低血清中α-fodrin的浓度，SMG中TNF-α和IL-6的表达，并下调IL-17A，TNF-α，CXCL9，CXCL13和BAFF的mRNA表达以及IL-17A和IL-6的蛋白表达。 SMG中的BAFF。给药16周后，TGP治疗可降低血清SSA / Ro，SSB / La和α-fodrin浓度，并降低BAFF蛋白表达和TNF-α，CXCL9，CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。TGP治疗可降低血清中α-fodrin的浓度，SMG中TNF-α和IL-6的表达，并下调IL-17A，TNF-α，CXCL9，CXCL13和BAFF的mRNA表达以及IL-17A和IL-6的蛋白表达。 SMG中的BAFF。给药16周后，TGP治疗可降低血清SSA / Ro，SSB / La和α-fodrin浓度，并降低BAFF蛋白表达和TNF-α，CXCL9，CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。CXCL13，IL-17A和BAFF mRNA表达。结论TGP通过抑制炎症反应对SS小鼠和NOD小鼠具有一定的治疗作用。
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