The increase in human infertility prevalence due to male reproductive disorders has been associated with extensive endocrine-disrupting chemical (EDC) exposure. Acrylamide (AA) is a compound formed spontaneously during heat processing of some foods that are mainly consumed by children and adolescents. In this study, we evaluated the prepubertal AA exposure effects on male adult reproductive physiology using a prepubertal experimental model to analyze the pubertal development, spermatogenesis hormones levels and genes expression involved in male reproductive function. This study is the first one to use the validated protocol to correlate the AA exposure with puberty development, as well as the AA-induced endocrine disrupting effects on reproductive axis. AA did not affect the age at puberty, the reproductive organ’s weight and serum hormonal levels. AA reduces spermatogenesis, induces morphological and functional defects on sperm and alters transcript expression of sexual hormone receptors (Ar and Esr2), the transcript expression of Tnf, Egr2, Rhcg and Lrrc34. These findings suggest that excessive AA consumption may impair their reproductive capacity at adulthood, despite no changes in hormonal profile being observed.

由于男性生殖障碍导致的人类不育患病率增加与广泛的内分泌干扰化学物质（EDC）暴露有关。丙烯酰胺（AA）是一些食物的加热加工过程中自发形成的化合物，这些食物主要由儿童和青少年食用。在这项研究中，我们使用青春期前的实验模型来评估青春期前AA暴露对男性成年生殖生理的影响，以分析青春期发育，精子生成激素水平和涉及男性生殖功能的基因表达。这项研究是第一个使用经过验证的方案将AA暴露与青春期发育以及AA诱导的对生殖轴的内分泌干扰作用相关的研究。AA不会影响青春期的年龄，生殖器官的重量和血清激素水平。Ar和Esr2），Tnf，Egr2，Rhcg和Lrrc34的转录表达。这些发现表明，尽管未观察到激素变化，但过量摄入AA可能会损害其成年后的生殖能力。