The extracellular matrix represents a dynamic microenvironment regulating essential cell functions in vivo. Tissue engineering approaches aim to recreate the native niche in vitro using biological scaffolds generated by organ decellularization. So far, the organ specific origin of such scaffolds was less considered and potential consequences for in vitro cell culture remain largely elusive. Here, we show that organ specific cues of biological scaffolds affect cellular behavior. In detail, we report on the generation of a well-preserved pancreatic bioscaffold and introduce a Scoring system allowing standardized inter-study quality assessment. Using multiple analysis tools for in-depth-characterization of the biological scaffold, we reveal unique compositional, physico-structural, and biophysical properties. Finally, we prove the functional relevance of the biological origin by demonstrating a regulatory effect of the matrix on the multi-lineage differentiation behavior of human induced pluripotent stem cells emphasizing the significance of matrix specificity for cellular behavior in artificial microenvironments.

细胞外基质代表动态的微环境调节体内必需的细胞功能。组织工程方法旨在利用器官脱细胞产生的生物支架在体外重建天然生态位。到目前为止，此类支架的器官特异性起源尚未得到考虑，并且可能对体外产生影响细胞培养仍然难以捉摸。在这里，我们表明生物支架的器官特定提示会影响细胞行为。详细地，我们报告了保存完好的胰腺生物支架的产生，并引入了评分系统，该评分系统可进行标准化的研究间质量评估。使用多种分析工具对生物支架进行深入表征，我们揭示了独特的组成，物理结构和生物物理特性。最后，我们通过证明基质对人类诱导的多能干细胞的多谱系分化行为的调节作用，强调了基质特异性对于人工微环境中细胞行为的重要性，证明了生物学起源的功能相关性。