Self-powered sensor is considered as a promising, rapid, portable and miniaturized detection device that can work without external power input. In this work, a novel dual-photoelectrode self-powered aptasensor for digoxin detection was designed on the basis of a photofuel cell (PFC) composed of a black TiO₂ (B–TiO₂) photoanode and a CuBr photocathode in a single-chamber cell. The sensing platform avoided the use of membrane, free mediator, bioactive components and costly metal Pt electrodes. The large inherent bias between the Fermi energy level of B–TiO₂ and that of CuBr improved the electricity output of PFC that the open circuit potential (OCP) and the maximum power density (P_max) reached 0.58 V and 6.78 μW cm^-2 respectively. Based on the excellent output of PFC, digoxin aptamer was immobilized on photoanode as the recognition element to capture digoxin molecules, which realized the high sensitive and selective detection of digoxin. The self-powered aptasensor displayed a broad linear in the range from 10^-12 M to 10^-5 M with a detection limit (3 S/N) of 0.33 pM. This work paved a luciferous way for further rapid, portable, miniaturized and on-site self-powered sensors.

自供电传感器被认为是一种有前途，快速，便携式和小型化的检测设备，无需外部电源即可工作。在这项工作中，基于由黑色TiO ₂（B–TiO ₂）光电阳极和CuBr光电阴极组成的单室光电燃料电池（PFC），设计了一种新型的用于地高辛检测的双光电极自供电适体传感器。细胞。该传感平台避免了使用膜，自由介体，生物活性成分和昂贵的金属铂电极。B–TiO ₂的费米能级和CuBr的费米能级之间的大固有偏差提高了PFC的电输出，开路电势（OCP）和最大功率密度（P _max）分别达到0.58 V和6.78μWcm ^-2分别。基于PFC的优异输出，将地高辛适体固定在光阳极上作为捕获地高辛分子的识别元件，从而实现了对地高辛的高灵敏度和选择性检测。自供电适体传感器在10 ^-12  M至10 ^-5  M的范围内显示出较宽的线性，检测极限（3 S / N）为0.33 pM。这项工作为进一步快速，便携式，小型化和现场自供电传感器的开发铺平了道路。