BACKGROUND This study investigated the anticancer potential of the medicinal herb, Cleome droserifolia (CD), a local plant of the Arabian Peninsula. C. droserifolia is traditionally known for its rubefacient, anti-diabetic, anti-oxidant, and anti-inflammatory properties. METHODS Organic fractions of the aerial parts of Cleome droserifolia harvested from the Arabian Peninsula were tested in human breast and cervical cancer cell lines for their anticancer potential. This was accomplished by using biochemical and cellular assays, including MTT, caspase Glo, western blot, and annexin V/propidium iodide-based flow cytometry analyses. RESULTS Test of the dichloromethane fraction of the methanolic extract of C. droserifolia, (CDD) revealed potent cytotoxic activity (from 70 to 90%) against several human cancer cell lines, including MCF-7, MDA-MB-231, and HeLa. Further characterization of the CDD fraction in MCF-7 cells revealed that it could activate the enzymatic activity of various caspases in a statistically significant manner, and induce cleavage of both caspase 7 and poly ADB ribose polymerase (PARP) proteins, but not the ethyl acetate fraction. Test of the ability of CDD to induce early signs of apoptosis was validated by annexin V/propidium iodide assay using FACS analysis. Induction of apoptosis was completely reversed by the classic pan inhibitor of apoptosis, Z-VAD-FMK, reducing early apoptosis from 29.7 to 0.6%, confirming that CDD could induce caspase-dependent apoptosis. CONCLUSIONS Altogether, our results reveal that C. droserifolia is a valuable medicinal plant with bioactive molecules that can induce apoptosis in human cancer cells. Thus, this plant should be explored further for its potential as an anticancer natural therapy as well as the isolation of novel molecules with anticancer properties.

中文翻译：

---

Cleome droserifolia的有机提取物表现出有效的caspase依赖性抗癌活性。

背景技术这项研究调查了阿拉伯半岛的本地植物药用草药Cleome droserifolia（CD）的抗癌潜力。传统上，C。droserifolia以其有效的抗糖尿病，抗糖尿病，抗氧化和抗炎特性而闻名。方法在阿拉伯人和宫颈癌细胞系中检测从阿拉伯半岛收获的埃及醉蝶花地上部分有机成分的抗癌潜力。这是通过使用生化和细胞分析（包括MTT，半胱天冬酶Glo，western blot和基于膜联蛋白V /碘化丙啶的流式细胞术分析）完成的。结果测试了C. droserifolia（CDD）甲醇提取物的二氯甲烷馏分，发现其对几种人类癌细胞系（包括MCF-7，MDA-MB-231，和HeLa。在MCF-7细胞中CDD部分的进一步表征表明，它可以以统计学上显着的方式激活各种胱天蛋白酶的酶促活性，并诱导caspase 7和多聚ADB核糖聚合酶（PARP）蛋白的裂解，但不能裂解乙酸乙酯分数。通过膜联蛋白V /碘化丙啶分析，使用FACS分析验证了CDD诱导凋亡早期迹象的能力。经典的泛凋亡抑制剂Z-VAD-FMK完全逆转了细胞凋亡的诱导作用，将早期细胞凋亡从29.7％降低至0.6％，证实CDD可以诱导caspase依赖性细胞凋亡。结论总的来说，我们的结果表明，德氏假丝酵母是一种有价值的药用植物，其生物活性分子可以诱导人癌细胞的凋亡。从而，