Heterogeneity of oil-bearing formations is one of major contributors to low oil recovery efficiency globally. Long-term water flooding will aggravate this heterogeneity by resulting in many large channels during the exploitation process. Thus, injected water quickly flows through these large channels rather than oil-bearing areas, which ultimately leads to low oil recovery. This problem can be solved by profile control using polymer plugging. However, non-deep profile control caused by premature plugging is the main challenge. Here, a conditional bacterial cellulose-producing strain, namely Enterobacter sp. FY-0701, was constructed for deep profile control to solve the problem of premature plugging. Its deep profile control and oil displacement capabilities were subsequently identified and assessed. The conditional bacterial cellulose-producing strain Enterobacter sp. FY-0701 was constructed by knocking out a copy of fructose-1, 6-bisphosphatase (FBP) encoding gene in Enterobacter sp. FY-07. Scanning electron microscope observation showed this strain produced bacterial cellulose using glucose rather than glycerol as the sole carbon source. Bacterial concentration and cellulose production at different locations in core experiments indicated that the plugging position of FY-0701 was deeper than that of FY-07. Moreover, enhanced oil recovery by FY-0701 was 12.09%, being 3.86% higher than that by FY-07 in the subsequent water flooding process. To our knowledge, this is the first report of conditional biopolymer-producing strains used in microbial enhance oil recovery (MEOR). Our results demonstrated that the conditional bacterial cellulose-producing strain can in situ produce biopolymer far from injection wells and plugs large channels, which increased the sweep volume of injection water and enhance oil recovery. The construction of this strain provides an alternative strategy for using biopolymers in MEOR.

中文翻译：

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通过使用产自肠杆菌属的条件细菌纤维素生产菌株进行深度剖面控制，微生物可提高采油率。07财年。

含油地层的非均质性是导致全球低采油效率的主要因素之一。长期注水将在开发过程中形成许多大通道，从而加剧这种异质性。因此，注入的水迅速流过这些大通道，而不是流经含油区域，最终导致采油率低。该问题可以通过使用聚合物封堵的轮廓控制来解决。然而，由过早堵塞引起的非深度剖面控制是主要挑战。这里，产生条件细菌纤维素的菌株，即肠杆菌属。FY-0701用于深度剖面控制，以解决过早堵塞的问题。随后确定并评估了其深剖面控制和驱油能力。条件性细菌纤维素生产菌株肠杆菌属。FY-0701是通过敲除肠杆菌属细菌中果糖-1、6-双磷酸酶（FBP）编码基因的副本而构建的。07财年。扫描电子显微镜观察表明，该菌株利用葡萄糖而不是甘油作为唯一碳源产生细菌纤维素。核心实验中不同位置的细菌浓度和纤维素产量表明，FY-0701的堵塞位置比FY-07的堵塞位置更深。此外，在随后的注水过程中，FY-0701的采收率提高了12.09％，比FY-07提高了3.86％。据我们所知，这是微生物增强油采收率（MEOR）中使用的条件性生物聚合物生产菌株的首次报道。我们的结果表明，有条件的细菌纤维素生产菌株可以在远离注入井的地方原位生产生物聚合物，并堵塞了大通道，从而增加了注入水的波及量并提高了石油采收率。该菌株的构建为在MEOR中使用生物聚合物提供了另一种策略。