Lipid-Lowering Effects and Intestinal Transport of Polyphenol Extract from Digested Buckwheat in Caco-2/HepG2 Coculture Models,Journal of Agricultural and Food Chemistry

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Lipid-Lowering Effects and Intestinal Transport of Polyphenol Extract from Digested Buckwheat in Caco-2/HepG2 Coculture Models
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-03-26 , DOI: 10.1021/acs.jafc.0c00321
Yijun Yao _{1,

2} , Feiran Xu ₁ , Xingrong Ju _{1,

2} , Zhifang Li ₂ , Lifeng Wang ₂

Affiliation

Polyphenol extracts derived from gastrointestinal digestates of buckwheat (Fagopyrum Mill) were studied for their intestinal transport and lipid-lowering effects in Caco-2/HepG2 coculture models. The relative amounts of all phenolic compounds throughout the digestion and intestinal absorption process were determined by UHPLC-Q-Orbitrap mass spectrometry. The digestible and easily transported phenolic compounds in buckwheat extract were identified. Herein, four main phenolic compounds and their metabolites were found on both the apical and basolateral sides of the Caco-2 cell transwell model. The transepithelial transport rates in the Caco-2 cell monolayer were scoparone (0.97) > hydroxycinnamic acid (0.40) > rutin (0.23) > quercetin (0.20). The main metabolism of hydroxycinnamic acid, quercetin, and scoparone in transepithelial transport was found to be methylation. Furthermore, results indicated that triglyceride, low-density lipoprotein cholesterol, total cholesterol, aspartate aminotransferase, and alanine aminotransferase levels in HepG2 cells on the basolateral side of coculture models can be suppressed by 53.64, 23.44, 36.49, 27.98, and 77.42% compared to the oleic acid-induced group (p < 0.05). In addition, the mRNA expression of Fabp4 relative to the control was found to be significantly upregulated (85.82 ± 10.64 to 355.18 ± 65.83%) by the easily transported buckwheat polyphenol components in HepG2 cells (p < 0.01).

中文翻译：

消化荞麦中多酚提取物在Caco-2 / HepG2共培养模型中的降脂作用和肠运输

研究了在Caco-2 / HepG2共培养模型中从荞麦的消化道中提取的多酚提取物（Fagopyrum Mill）的肠道运输和降脂作用。通过UHPLC-Q-Orbitrap质谱法测定整个消化和肠道吸收过程中所有酚类化合物的相对量。鉴定了荞麦提取物中可消化且易于运输的酚类化合物。在此，在Caco-2细胞转运模型的顶端和基底外侧均发现了四种主要的酚类化合物及其代谢产物。Caco-2细胞单层中的上皮转运速率为可可酮（0.97）>羟基肉桂酸（0.40）>芦丁（0.23）>槲皮素（0.20）。羟基肉桂酸，槲皮素，发现上皮转运中的可卡酮是甲基化的。此外，结果表明，与共培养模型相比，共培养模型底部外侧HepG2细胞中的甘油三酸酯，低密度脂蛋白胆固醇，总胆固醇，天冬氨酸转氨酶和丙氨酸转氨酶水平可被抑制53.64％，23.44、36.49、27.98和77.42％。油酸诱导的基团（p＜0.05）。此外，通过在HepG2细胞中容易运输的荞麦多酚成分，发现Fabp4相对于对照的mRNA表达显着上调（85.82±10.64至355.18±65.83％）（p <0.01）。

更新日期：2020-03-27

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