Micromotor-Based Biosensing Using Directed Transport of Functionalized Beads.,ACS Sensors

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Micromotor-Based Biosensing Using Directed Transport of Functionalized Beads.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-03-06 , DOI: 10.1021/acssensors.9b02041
Sinwook Park ₁ , Gilad Yossifon ₁

Affiliation

Previous micromotor-based biosensing studies used to functionalize the surface of the micromotor with specific molecular probes for binding of target analyte, thus limiting the use of the micromotor for the specific target. In contrast, here, we introduce a novel approach of using a nonfunctionalized micromotor as a generic cargo carrier being able to perform label-free and dynamic loading, transport, and release of functionalized beads. Hence, such an approach enables one to use the same micromotor system for sensing of varying targets via different commercially available functionalized beads, demonstrating the use of micromotors as a practical and versatile means for biosensing. We have also introduced a simplified microfluidic design that can be used for immunosensing or DNA binding tests without necessity for complicated fluid handling (buffer exchange, washing, etc.) steps. We expect this approach to open up new realizations of simplified and generic biosensing platforms.

中文翻译：

基于微电机的生物传感，使用功能化微珠的直接运输。

以前的基于微马达的生物传感研究曾使用特定的分子探针对微马达的表面进行功能化，以结合目标分析物，从而限制了微马达对特定目标的使用。相比之下，在这里，我们介绍一种使用非功能化微电机作为通用货物载体的新颖方法，该载运器能够执行无标签且动态加载，运输和释放功能化微珠。因此，这种方法使人们能够使用相同的微电机系统通过不同的可商购的功能化珠粒来感测变化的靶标，证明了微电机作为生物传感的实用且通用的手段的使用。我们还介绍了一种简化的微流体设计，该设计可用于免疫传感或DNA结合测试，而无需进行复杂的流体处理（缓冲液交换，洗涤等）步骤。我们希望这种方法将为简化和通用的生物传感平台开辟新的认识。

更新日期：2020-03-06

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