A method is described for preparation of monosubstituted derivatives of platinum and palladium coproporphyrin-I. The method is based on chemical differentiation of one of the four alkoxycarbonyl groups of the starting coproporphyrin-I tetraalkyl ester by selective acid hydrolysis. The hydrolysis results after chromatographic purification to pure mono hydroxycarbonyl coproporphyrin, which can be subsequently coupled with a chemical species carrying a functional group of interest. The described method is used for preparation of phosphorescent metalloporphyrin labeling reagents having an isothiocyanato reactive group.

中文翻译：

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单功能和磷光钯(II)和铂(II)粪卟啉标记试剂的制备

描述了一种制备铂和钯粪卟啉-I的单取代衍生物的方法。该方法基于通过选择性酸水解对起始粪卟啉-I四烷基酯的四个烷氧基羰基之一进行化学分化。色谱纯化后水解为纯单羟基羰基粪卟啉，随后可与带有目的官能团的化学物质偶联。所述方法用于制备具有异硫氰酸根反应基团的磷光金属卟啉标记试剂。