Methods for in vitro modeling of glioma invasion: Choosing tools to meet the need.,Glia

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Methods for in vitro modeling of glioma invasion: Choosing tools to meet the need.
Glia ( IF 5.4 ) Pub Date : 2020-03-05 , DOI: 10.1002/glia.23813
Bilge Dundar ₁ , Steven M Markwell ₂ , Nitya V Sharma ₂ , Cheryl L Olson ₂ , Subhas Mukherjee ₂ , Daniel J Brat ₂

Affiliation

Widespread tumor cell invasion is a fundamental property of diffuse gliomas and is ultimately responsible for their poor prognosis. A greater understanding of basic mechanisms underlying glioma invasion is needed to provide insights into therapies that could potentially counteract them. While none of the currently available in vitro models can fully recapitulate the complex interactions of glioma cells within the brain tumor microenvironment, if chosen and developed appropriately, these models can provide controlled experimental settings to study molecular and cellular phenomena that are challenging or impossible to model in vivo. Therefore, selecting the most appropriate in vitro model, together with its inherent advantages and limitations, for specific hypotheses and experimental questions achieves primary significance. In this review, we describe and discuss commonly used methods for modeling and studying glioma invasion in vitro, including platforms, matrices, cell culture, and visualization techniques, so that choices for experimental approach are informed and optimal.

中文翻译：

胶质瘤侵袭的体外建模方法：选择满足需要的工具。

广泛的肿瘤细胞侵袭是弥漫性胶质瘤的基本特性，最终导致其预后不良。需要更深入地了解胶质瘤侵袭的基本机制，以深入了解可能抵消它们的疗法。虽然目前可用的体外模型都不能完全概括脑肿瘤微环境中神经胶质瘤细胞的复杂相互作用，但如果选择和开发得当，这些模型可以提供受控的实验设置来研究具有挑战性或不可能建模的分子和细胞现象体内。因此，针对特定的假设和实验问题，选择最合适的体外模型及其固有的优势和局限性具有重要意义。在这次审查中，

更新日期：2020-03-05

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