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Correlation of hyperpolarized 13C‐MRI data with tissue extract measurements
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-03-04 , DOI: 10.1002/nbm.4269 Casey Y. Lee 1, 2 , Justin Y. C. Lau 1, 2, 3 , Benjamin J. Geraghty 1, 2 , Albert P. Chen 4 , Yi‐Ping Gu 2 , Charles H. Cunningham 1, 2
NMR in Biomedicine ( IF 2.7 ) Pub Date : 2020-03-04 , DOI: 10.1002/nbm.4269 Casey Y. Lee 1, 2 , Justin Y. C. Lau 1, 2, 3 , Benjamin J. Geraghty 1, 2 , Albert P. Chen 4 , Yi‐Ping Gu 2 , Charles H. Cunningham 1, 2
Affiliation
|
Hyperpolarized (HP) 
MRI provides the means to monitor lactate metabolism noninvasively in tumours. Since 
‐lactate signal levels obtained from HP 
imaging depend on multiple factors, such as the rate of 
substrate delivery via the vasculature, the expression level of monocarboxylate transporters (MCTs) and lactate dehydrogenase (LDH), and the local lactate pool size, the interpretation of HP 
metabolic images remains challenging. In this study, ex vivo tissue extract measurements (i.e., NMR isotopomer analysis, western blot analysis) derived from an MDA‐MB‐231 xenograft model in nude rats were used to test for correlations between the in vivo 
data and the ex vivo measures. The lactate‐to‐pyruvate ratio from HP 
MRI was strongly correlated with [1‐
]lactate concentration measured from the extracts using NMR (R = 0.69, p
0.05), as well as negatively correlated with tumour wet weight (R = 
0.60, p
0.05). In this tumour model, both MCT1 and MCT4 expressions were positively correlated with wet weight (
= 0.78 and 0.93, respectively, p
0.01). Lactate pool size and the lactate‐to‐pyruvate ratio were not significantly correlated.
中文翻译：
超极化13C‐MRI数据与组织提取物测量值的相关性
超极化（HP）MRI提供了无创监测肿瘤中乳酸代谢的方法。由于从HP成像获得的乳酸信号水平取决于多种因素，例如通过脉管系统传递底物的速率，单羧酸盐转运蛋白（MCTs）和乳酸脱氢酶（LDH）的表达水平以及局部乳酸池的大小，因此HP代谢图像仍然具有挑战性。在这项研究中，使用来自裸鼠MDA-MB-231异种移植模型的离体组织提取物测量值（即NMR同位素异构体分析，蛋白质印迹分析）来测试体内数据与离体测量值之间的相关性。HP的乳酸/丙酮酸盐比率MRI与使用NMR从提取物中测得的[1- ]乳酸浓度高度相关
（R = 0.69，p
0.05），并且与肿瘤湿重呈负相关（R = 0.60，p
0.05）。在该肿瘤模型中，MCT1和MCT4的表达均与湿重呈正相关（
分别为0.78和0.93，p =
0.01）。乳酸池的大小和乳酸与丙酮酸的比例没有显着相关。
更新日期：2020-04-22
MRI provides the means to monitor lactate metabolism noninvasively in tumours. Since ‐lactate signal levels obtained from HP imaging depend on multiple factors, such as the rate of substrate delivery via the vasculature, the expression level of monocarboxylate transporters (MCTs) and lactate dehydrogenase (LDH), and the local lactate pool size, the interpretation of HP metabolic images remains challenging. In this study, ex vivo tissue extract measurements (i.e., NMR isotopomer analysis, western blot analysis) derived from an MDA‐MB‐231 xenograft model in nude rats were used to test for correlations between the in vivo data and the ex vivo measures. The lactate‐to‐pyruvate ratio from HP MRI was strongly correlated with [1‐
]lactate concentration measured from the extracts using NMR (R = 0.69, p
0.05), as well as negatively correlated with tumour wet weight (R = 0.60, p
0.05). In this tumour model, both MCT1 and MCT4 expressions were positively correlated with wet weight (
= 0.78 and 0.93, respectively, p
0.01). Lactate pool size and the lactate‐to‐pyruvate ratio were not significantly correlated.
中文翻译：
超极化13C‐MRI数据与组织提取物测量值的相关性
超极化（HP）
MRI提供了无创监测肿瘤中乳酸代谢的方法。由于从HP成像获得的乳酸信号水平取决于多种因素，例如通过脉管系统传递底物的速率，单羧酸盐转运蛋白（MCTs）和乳酸脱氢酶（LDH）的表达水平以及局部乳酸池的大小，因此HP代谢图像仍然具有挑战性。在这项研究中，使用来自裸鼠MDA-MB-231异种移植模型的离体组织提取物测量值（即NMR同位素异构体分析，蛋白质印迹分析）来测试体内数据与离体测量值之间的相关性。HP的乳酸/丙酮酸盐比率MRI与使用NMR从提取物中测得的[1- ]乳酸浓度高度相关
（R = 0.69，p
0.05），并且与肿瘤湿重呈负相关（R = 0.60，p
0.05）。在该肿瘤模型中，MCT1和MCT4的表达均与湿重呈正相关（
分别为0.78和0.93，p =
0.01）。乳酸池的大小和乳酸与丙酮酸的比例没有显着相关。
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