Photodynamic action of Hypericum perforatum hydrophilic extract against Staphylococcus aureus,Photochemical & Photobiological Sciences

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Photodynamic action of Hypericum perforatum hydrophilic extract against Staphylococcus aureus
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2020/03/05 , DOI: 10.1039/c9pp00428a
Pietro Delcanale _{1,

2,

3,

4} , Cormac Hally _{4,

5,

6,

7,

8} , Santi Nonell _{4,

5,

6,

7} , Silvia Bonardi _{9,

10,

11,

12} , Cristiano Viappiani _{8,

12,

13,

14,

15} , Stefania Abbruzzetti _{8,

12,

13,

14,

15}

Affiliation

Hypericin (Hyp) is one of the most effective, naturally occurring photodynamic agents, which proved effective against a wide array of microorganisms. One limitation of its large scale application as a disinfectant is the high production cost of the pure compound. The availability of photoactive materials at a lower cost may be highly beneficial to the actual implementation of photodisinfection also at the industrial level. In this work we report the use of a lyophilized extract from Hypericum perforatum as a photosensitizing material. We show that optical absorption in the green-red region of the visible spectrum of ethanol or DMSO solutions of the lyophilized extract contains bands arising from Hyp. When excited with light in the main Hyp absorption bands, fluorescence emission and triplet state formation occur as in pure Hyp solutions. We show that ethanol or DMSO solutions of the lyophilized extract from Hypericum perforatum are highly efficient photodynamic agents against Gram-positive Staphylococcus aureus, chosen as a model. The performance is indistinguishable from that of the pure compound. Using fluorescence microscopy, we demonstrate that upon incubation of S. aureus with lyophilized extract solutions, Hyp is found on the bacterial wall, as previously reported for the pure compound.

中文翻译：

贯叶连翘亲水提取物对金黄色葡萄球菌的光动力作用

金丝桃素（Hyp）是最有效的天然光动力剂之一，事实证明它能有效抵抗多种微生物。其作为消毒剂的大规模应用的局限性在于纯化合物的高生产成本。甚至在工业水平上，以较低的成本获得的光活性材料对于光消毒的实际实施可能是非常有益的。在这项工作中，我们报告了贯叶连翘的冻干提取物的使用作为光敏材料。我们表明，冻干提取物的乙醇或DMSO溶液的可见光谱的绿红色区域中的光吸收包含Hyp产生的谱带。当在主要Hyp吸收带中被光激发时，会像在纯Hyp溶液中一样发生荧光发射和三重态形成。我们显示从贯叶连翘冻干提取物的乙醇或DMSO解决方案是针对革兰氏阳性金黄色葡萄球菌（选择为模型）的高效光动力剂。其性能与纯化合物没有区别。使用荧光显微镜，我们证明金黄色葡萄球菌孵育后如先前报道的那样，用冻干的提取液在细菌壁上发现Hyp。

更新日期：2020-03-19

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