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Streptomyces acidicola sp. nov., isolated from a peat swamp forest in Thailand.
The Journal of Antibiotics ( IF 3.3 ) Pub Date : 2020-03-05 , DOI: 10.1038/s41429-020-0294-5
Kenika Lipun 1 , Tanatorn Chantavorakit 1 , Ratchanee Mingma 2 , Kannika Duangmal 1
Affiliation  

A novel actinobacterium, designated strain K1PN6T, was isolated from soil sample collected in Kantulee peat swamp forest, Surat Thani province, Thailand. The morphological, chemotaxonomic, and phylogenetic characteristics were consistent with its classification in the genus Streptomyces. Based on 16S rRNA gene sequence analysis, strain K1PN6T showed highest similarity to Streptomyces phyllanthi PA1-07T (98.6 %), Streptomyces spongiae Sp080513SC-24T (98.3%) and Streptomyces adustus WH-9T (98.3%). The G + C content of the genomic DNA was 70.3 mol%. Digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain K1PN6T with S. phyllanthi TISTR 2346T (33.7 and 89.1%), S. spongiae NBRC 106415T (38.6 and 90.6%) and S. adustus NBRC 109810T (26.0 and 86.2%) were below the thresholds of 70 and 95–96% for prokaryotic conspecific assignation. Chemotaxonomic data revealed that strain K1PN6T possessed MK-9(H8) (45%) and MK-9(H6) (34%) as the predominant menaquinones. It contained ll-diaminopimelic acid as the diagnostic diamino acid and galactose, glucose, mannose, and ribose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unidentified aminolipids, an unidentified phospholipid, and glycophospholipid. The predominant cellular fatty acids (>10%) were iso-C16:0, C16:0, anteiso-C15:0, and iso-C14:0. On the basis of these genotypic and phenotypic data, strain K1PN6T should be designated as a representative of a novel species of the genus Streptomyces, for which the name Streptomyces acidicola sp. nov. is proposed with the type strain K1PN6T (=TBRC 11341T=NBRC 114304T).



中文翻译:

酸性链霉菌 十一月。,与泰国的泥炭沼泽森林隔离。

一种新颖的actinobacterium,指定应变K1PN6 Ť,从在Kantulee泥炭沼泽森林采集的土壤样品,索叻他尼省,泰国分离。链霉菌属的形态,化学分类和系统发育特征与其分类一致。根据16S rRNA基因序列分析,菌株K1PN6 T花链链霉菌PA1-07 T(98.6%),海绵链霉菌Sp080513SC-24 T(98.3%)和链霉菌WH-9 T相似性最高。(98.3%)。基因组DNA的G + C含量为70.3mol%。数字DNA-DNA杂交和应变K1PN6的基因组序列之间的平均核苷酸同一性值ŤS.余甘子TISTR 2346 Ť(33.7和89.1%),S。spongiae NBRC 106415 Ť(38.6和90.6%)和S. adustus NBRC 109810 T(26.0和86.2%)低于原核同种分配的阈值70和95–96%。化学分类数据显示,菌株K1PN6 T具有MK-9(H 8)(45%)和MK-9(H 6)(34%)作为主要的甲萘醌。它包含ll-二氨基庚二酸作为诊断性二氨基酸,半乳糖,葡萄糖,甘露糖和核糖为全细胞糖。极性脂质包括二磷脂酰甘油,磷脂酰乙醇胺,磷脂酰甘油,磷脂酰肌醇,磷脂酰肌醇甘露糖苷,两个未鉴定的氨基脂质,未鉴定的磷脂和糖脂。主要的细胞脂肪酸(> 10%)是iso-C 16:0,C 16:0,anteiso-C 15:0和iso-C 14:0。根据这些基因型和表型数据,应将菌株K1PN6 T指定为链霉菌属新物种的代表,其名称为酸性链霉菌sp。十一月 建议使用类型为K1PN6 T(= TBRC 11341 T = NBRC 114304 T)的应变。

更新日期:2020-03-05
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