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An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study.
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.1 ) Pub Date : 2020-03-05 , DOI: 10.1016/j.jpba.2020.113235 Li-Feng Li 1 , Tin-Long Wong 1 , Ji-Xia Zhang 2 , Li-Shuang Zhou 1 , Su-Ping Bai 2 , Hau-Yee Fung 1 , Hui-Yuan Cheng 1 , Quan-Wei Zhang 1 , Hong-Ming Zheng 1 , Wan-Rong Bao 1 , Dik-Lung Ma 3 , Chung-Hang Leung 4 , Ge Zhang 1 , Zhao-Xiang Bian 1 , Ai-Ping Lyu 1 , Quan-Bin Han 1
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.1 ) Pub Date : 2020-03-05 , DOI: 10.1016/j.jpba.2020.113235 Li-Feng Li 1 , Tin-Long Wong 1 , Ji-Xia Zhang 2 , Li-Shuang Zhou 1 , Su-Ping Bai 2 , Hau-Yee Fung 1 , Hui-Yuan Cheng 1 , Quan-Wei Zhang 1 , Hong-Ming Zheng 1 , Wan-Rong Bao 1 , Dik-Lung Ma 3 , Chung-Hang Leung 4 , Ge Zhang 1 , Zhao-Xiang Bian 1 , Ai-Ping Lyu 1 , Quan-Bin Han 1
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Polysaccharides have broad bioactivities and are major components of water decoction of herb formulae. However, the quality control of polysaccharides remains a challenge. Oligosaccharide-fragment approach has been considered in elucidating chemical structures of polysaccharides, but never been used for quantitation. Using reference chemicals and a real sample Danggui Buxue Tang (DBT) in this study, an oligosaccharide-marker approach was established to quantify specific polysaccharides. Firstly, linear relationships between parent polysaccharides and hydrolysis-produced daughter oligosaccharides were verified using reference polysaccharides. Then in case of DBT, two fluorescence-labeled oligosaccharides with high specificity to individual parent polysaccharides were selected as markers. They were easily isolated and identified. Their potential in quantification of parent polysaccharides were satisfactorily validated in terms of linearity (r≥0.99), repeatability (RSD ≤ 8.4 %), and spike recovery (≥80 %). This method could be a promising approach for quality assessment of polysaccharides in herbal formulae.
中文翻译：
LC-qTOF-MS定量分析草药配方中特定多糖的寡糖标记方法：当归补血汤，一个案例研究。
多糖具有广泛的生物活性，是草药配方水煎剂的主要成分。但是，多糖的质量控制仍然是一个挑战。在阐明多糖的化学结构时曾考虑使用寡糖片段化方法，但从未用于定量。在本研究中，使用参考化学品和真实样品当归补血汤（DBT），建立了寡糖标记方法来定量特定多糖。首先，使用参考多糖验证母体多糖和水解产生的子寡糖之间的线性关系。然后在DBT的情况下，选择对单个母体多糖具有高度特异性的两种荧光标记的寡糖作为标记。它们很容易被隔离和识别。通过线性度（r≥0.99），重复性（RSD≤8.4％）和加标回收率（≥80％）令人满意地验证了它们在母体多糖定量中的潜力。这种方法可能是一种有前景的草药配方中多糖质量评估的方法。
更新日期：2020-03-05
中文翻译：
LC-qTOF-MS定量分析草药配方中特定多糖的寡糖标记方法：当归补血汤，一个案例研究。
多糖具有广泛的生物活性，是草药配方水煎剂的主要成分。但是，多糖的质量控制仍然是一个挑战。在阐明多糖的化学结构时曾考虑使用寡糖片段化方法，但从未用于定量。在本研究中，使用参考化学品和真实样品当归补血汤（DBT），建立了寡糖标记方法来定量特定多糖。首先，使用参考多糖验证母体多糖和水解产生的子寡糖之间的线性关系。然后在DBT的情况下，选择对单个母体多糖具有高度特异性的两种荧光标记的寡糖作为标记。它们很容易被隔离和识别。通过线性度（r≥0.99），重复性（RSD≤8.4％）和加标回收率（≥80％）令人满意地验证了它们在母体多糖定量中的潜力。这种方法可能是一种有前景的草药配方中多糖质量评估的方法。
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