The increasing application of approaches that allow tracing of individual cells over time, together with transcriptomics and epigenomics analyses is changing the way resident stromal stem cells (mesenchymal stem cells) are viewed. Rather than being a defined, homogeneous cell population as described following in vitro expansion, in vivo, these cells are highly programmed according to their resident tissue location. This programming is evidenced by different epigenetic landscapes and gene transcription signatures in cells before any in vitro expansion. This has potentially profound implications for the heterotypic use of these cells in therapeutic tissue engineering applications.

中文翻译：

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驱动间充质干细胞谱系承诺的表观遗传机制

随着时间的推移追踪单个细胞的方法越来越多地应用，连同转录组学和表观基因组学分析，正在改变对驻留基质干细胞（间充质干细胞）的看法。这些细胞不是在体外扩增后所描述的确定的、同质的细胞群，而是根据它们的常驻组织位置进行高度编程的。在任何体外扩增之前，细胞中不同的表观遗传景观和基因转录特征证明了这种编程。这对这些细胞在治疗性组织工程应用中的异型使用具有潜在的深远意义。