Bioimaging carried out using two or more fluorophores possessing different emission wavelength can be termed as a multicolor/multiplexed bioimaging technique. Traditionally, images are captured sequentially using multiple fluorophores having specific excitation and emission. For this purpose, multifunctional nanoprobes, such as organic fluorophores, metallic nanoparticles, semiconductor quantum dots, and carbon dots (CDs) are used. Among these fluorophores, quantum dots (QDs) have emerged as an ideal probe for multiplexed bioimaging due to their unique property of size tunable emission. However, the usage of quantum dots in bioimaging is limited due to their toxicity. Furthermore, the reproducibility of optical properties is cynical. These desirable properties, along with enhancement in quantum efficiency, photostability, fluorescence lifetime, etc. can be achieved using precision control over synthesis parameters. This review summarizes the desirable properties and synthesis methods of such superior QDs followed by their application in multiplexed imaging.

使用具有不同发射波长的两种或更多种荧光团进行的生物成像可以被称为多色/多重生物成像技术。传统上，使用具有特定激发和发射的多种荧光体顺序捕获图像。为此，使用多功能纳米探针，例如有机荧光团，金属纳米颗粒，半导体量子点和碳点（CD）。在这些荧光团中，量子点（QD）由于其大小可调发射的独特特性而成为多路生物成像的理想探针。但是，量子点在生物成像中的使用由于其毒性而受到限制。此外，光学性质的再现性是愤世嫉俗的。这些理想的特性以及增强的量子效率，光稳定性，荧光寿命，可以通过对合成参数进行精确控制来实现等。这篇综述总结了这种优质量子点的理想性质和合成方法，然后将其应用于多路成像。