Abstract A ceramic filter medium (CFM) and dewatered aluminum sludge (DAS) were used as a small-scale combined biofilter to treat synthetic livestock wastewater. The average removal efficiency of ammonia nitrogen (NH4+-N) and total nitrogen (TN) reached 95.75 and 75.11% respectively. Illumina sequencing indicated that, except for initial media, most beta diversities of napA, narG, nirS, qnorB, and nosZ in the CFM or DAS were in a steady-state condition regardless of space-time variation, while these genes showed discrepancy between the CFM and DAS. In addition, the dominant genera were principally influenced by the synergic environmental factors, namely NH4+-N, TN, chemical oxygen demand (COD), and pH of effluents. This research demonstrated that the elimination of TN for treating synthetic livestock wastewater could be achieved by increasing the relative abundance of Azoarcus, Rhodanobacter, and Burkholderia. Meanwhile, increasing these genera contributes to suppressing the genera disadvantageous to TN attenuation.

中文翻译：

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新型生物反应器脱氮处理模拟牲畜废水脱硝菌的响应

摘要 采用陶瓷过滤介质（CFM）和脱水铝污泥（DAS）作为小型组合生物过滤器处理合成牲畜废水。氨氮(NH4+-N)和总氮(TN)的平均去除率分别达到95.75%和75.11%。Illumina测序表明，除初始培养基外，CFM或DAS中napA、narG、nirS、qnorB和nosZ的大多数β多样性均处于稳态状态，而与时空变化无关，而这些基因在CFM 和 DAS。此外，优势属主要受协同环境因素的影响，即NH4+-N、TN、化学需氧量（COD）和出水pH值。该研究表明，通过增加固氮弧菌、红丹诺杆菌和伯克霍尔德菌的相对丰度，可以消除用于处理合成牲畜废水的 TN。同时，增加这些属有助于抑制对TN衰减不利的属。