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Study of the effect of cypermethrin on the spider Polybetes phytagoricus in different energy states
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-05-01 , DOI: 10.1016/j.pestbp.2020.104559
A Laino 1 , C F Garcia 1
Affiliation  

Spiders are found among the most important predators of plague insects of numerous agricultural systems. They are the most numerous representatives of the Class Arachnid and are widely distributed in numerous ecosystems. Due to multiple variables, living beings are exposed to quantitative transitions of their energetic reserves, which affect their sensitivity before the different xenobiotics. In the present study we evaluate the effect of cypermethrin (pyrethroid) on different metabolic/energetic stages of the spider Polybetes pythagoricus (Sparassidae). We firstly studied LD50 of cypermethrin on young, males and, pre-vitellogenic and post-vitellogenic females. The activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione-S-transferase (GST) and acetylcholinesterase (AChE) was analyzed. Results indicate that young have a higher caloric content compared to adults, females have a higher caloric content than males and vitellogenesis generates a great calory decrease in females. The LD50 was significantly lower in young (10%) (103 ng/g weight) in relation to the three models of adults (969-1108 ng/g weight). Vitellogenesis causes an increase of free radicals as a result of the different metabolic processes which manifest as an increase in the lipid peroxidation. Doses at the LD30 and LD40 levels of cypermethrin did not generate alterations in any of the enzymes analyzed in young, this fact may probably provoke an increase of lipid peroxidation (evaluated as a great MDA increase). The activity of the enzymes linked to oxidative stress was altered by this doses in the three adult models, the enzymatic activity CAT, GR, and GST was sex-dependent. Post-vitellogenic females showed a greater activity of CAT, SOD, GST and GR before the xenobiotics than pre-vitellogenic ones, probably as a consequence of metabolic stress generated during vitellogenesis.

中文翻译:

氯氰菊酯对不同能态蜘蛛Polybetes phytagoricus的影响研究

在众多农业系统中,蜘蛛是鼠疫昆虫最重要的捕食者之一。它们是蛛形纲中数量最多的代表,广泛分布于众多生态系统中。由于存在多种变量,生物暴露于其能量储备的数量变化,这会影响它们在不同外源生物之前的敏感性。在本研究中,我们评估了氯氰菊酯(拟除虫菊酯)对蜘蛛 Polybetes pythagoricus (Sparassidae) 不同代谢/能量阶段的影响。我们首先研究了氯氰菊酯对年轻、雄性、卵黄生成前和卵黄生成后雌性的 LD50。分析了超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽还原酶 (GR)、谷胱甘肽-S-转移酶 (GST) 和乙酰胆碱酯酶 (AChE) 的活性。结果表明,与成年人相比,年轻人的热量含量更高,女性的热量含量高于男性,并且卵黄生成使女性的热量大幅减少。与三种成人模型(969-1108 ng/g 体重)相比,年轻人(10%)(103 ng/g 体重)的 LD50 显着较低。作为不同代谢过程的结果,卵黄生成导致自由基的增加,表现为脂质过氧化的增加。在 LD30 和 LD40 水平的氯氰菊酯剂量不会在年轻人中分析的任何酶中产生改变,这一事实可能会引起脂质过氧化的增加(被评估为 MDA 的显着增加)。在三个成人模型中,与氧化应激相关的酶的活性因该剂量而改变,酶活性 CAT、GR、和 GST 与性别有关。卵黄生成后雌性在异生素之前表现出更高的 CAT、SOD、GST 和 GR 活性,这可能是卵黄生成过程中产生的代谢压力的结果。
更新日期:2020-05-01
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